Anti-CD8抗体[RFT-8] (PE/Cy7 ®) (ab25389)


  • 产品名称Anti-CD8抗体[RFT-8] (PE/Cy7 ®)
    参阅全部 CD8 一抗
  • 描述
    小鼠单克隆抗体[RFT-8] to CD8 (PE/Cy7 ®)
  • 偶联物PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • 经测试应用适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.



Our Abpromise guarantee covers the use of ab25389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab154457-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.


  • 功能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 疾病相关Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 形式CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
  • 别名
    • CD8 antibody
    • CD8 antigen, alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8a antigen antibody
    • CD8a molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    see all

Anti-CD8 antibody [RFT-8] (PE/Cy7 ®) 图像

  • ab25389 staining CD8 in Human PBMCs by Flow Cytometry. The sample was incubated with the primary antibody (1/100 in PBS + 2% serum and 0.5 mM EDTA) for 25 minutes at 4°C. Gating Strategy: Lymphocytes.

    See Abreview

Anti-CD8 antibody [RFT-8] (PE/Cy7 ®) (ab25389)参考文献

ab25389 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Permeabilization No
Sample Human Cell (PBMC)
Specification PBMC
Gating Strategy lymphocytes
Preparation Cell harvesting/tissue preparation method: Ficoll
Sample buffer: DPBS

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提交于 Sep 17 2014