重组Anti-CD8 alpha抗体[EP1150Y] (ab93278)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1150Y] to CD8 alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD8 alpha抗体[EP1150Y]
参阅全部 CD8 alpha 一抗 -
描述
兔单克隆抗体[EP1150Y] to CD8 alpha -
宿主
Rabbit -
特异性
The immunogen sequence for this antibody has no significant homology with mouse and rat CD alpha and beta. This product showed specific staining in lymphocytes, although we observed background staining in some epithelial cells tested. -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human CD8 alpha aa 200 to the C-terminus (C terminal).
Database link: P01732 -
阳性对照
- WB: THP-1 cell lysate. ICC/IF: MOLT-4 cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1150Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab93278于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Predicted molecular weight: 26 kDa.
For unpurified use at 1/1000 - 1/10000. |
|
ICC/IF |
1/500.
For unpurified use at 1/50 - 1/100. |
说明 |
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WB
1/1000. Predicted molecular weight: 26 kDa. For unpurified use at 1/1000 - 1/10000. |
ICC/IF
1/500. For unpurified use at 1/50 - 1/100. |
靶标
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功能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
疾病相关
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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别名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
图片
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Immunocytochemistry analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) labeling CD8 alpha with purified ab93278 at 1/250 dilution (9 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.21 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
Anti-CD8 alpha antibody [EP1150Y] (ab93278) at 1/1000 dilution (unpurified) + THP-1 cell lysate at 10 µg
Predicted band size: 26 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (45)
ab93278 被引用在 45 文献中.
- Liu J et al. Multicenter phase II trial of Camrelizumab combined with Apatinib and Eribulin in heavily pretreated patients with advanced triple-negative breast cancer. Nat Commun 13:3011 (2022). PubMed: 35641481
- Lei X et al. Mitochondrial fission induces immunoescape in solid tumors through decreasing MHC-I surface expression. Nat Commun 13:3882 (2022). PubMed: 35794100
- Wu J et al. SPOP promotes cervical cancer progression by inducing the movement of PD-1 away from PD-L1 in spatial localization. J Transl Med 20:384 (2022). PubMed: 36042498
- Huo X et al. Identification of Novel Immunologic Checkpoint Gene Prognostic Markers for Ovarian Cancer. J Oncol 2022:8570882 (2022). PubMed: 36157232
- Zhu J et al. Photothermal Nano-Vaccine Promoting Antigen Presentation and Dendritic Cells Infiltration for Enhanced Immunotherapy of Melanoma via Transdermal Microneedles Delivery. Research (Wash D C) 2022:9816272 (2022). PubMed: 36157510