ab112219 reacts with CD8alpha found on Mouse thymocytes and Mouse cytotoxic/suppressor T cells. It does not bind to Mouse helper/inducer T cells. It binds to T lymphocytes from all Mouse strains regardless of phenotypic expression (i.e. reacts with T lymphocytes from mouse strains expressing the Ly 2.1 or Ly 2.2 phenotype).
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Antigen retrieval is required
Use 0.1µg for 106 cells. ab136125-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Handisurya A et al. Strain-specific properties and T cells regulate the susceptibility to papilloma induction by Mus musculus papillomavirus 1. PLoS Pathog10:e1004314 (2014).
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