Anti-CD8 alpha抗体[RPA-T8] (PE/Cy5®) (ab95595)

概述

  • 产品名称Anti-CD8 alpha抗体[RPA-T8] (PE/Cy5®)
    参阅全部 CD8 alpha 一抗
  • 描述
    小鼠单克隆抗体[RPA-T8] to CD8 alpha (PE/Cy5®)
  • 偶联物PE/Cy5®. Ex: 496nm, Em: 670nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    hPBMC stimulated with PHA.

  • 阳性对照
    • Normal Human peripheral blood cells

性能

应用

Our Abpromise guarantee covers the use of ab95595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 5µl for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 疾病相关Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [RPA-T8] (PE/Cy5®) 图像

  • Staining of normal Human peripheral blood cells with Mouse IgG1 isotype control PE-Cy5 (open histogram) or ab95595 (filled histogram). Cells in the lymphocyte gate were used for analysis.
  • ab95595 staining CD8 alpha in Human PBMCs cell by Flow Cytometry. Cells were harvested by Ficoll-hypaque gradient. The sample was incubated with the primary antibody (1/100 2% Human serum, 1mM EDTA in PBS) for 25 minutes at 4°C.
    Gating Strategy: Lymphocytes.

    See Abreview

Anti-CD8 alpha antibody [RPA-T8] (PE/Cy5®) (ab95595)参考文献

ab95595 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMC)
Specification PBMC
Preparation Cell harvesting/tissue preparation method: Ficoll-hypaque gradrient
Sample buffer: PBS
Permeabilization No
Gating Strategy lymphocytes
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提交于 Feb 19 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"