The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1.25µl for 106 cells. ab106163-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
疾病相关Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
ab95591 at 20 µl (0.5 µg)/per test staining normal human peripheral blood cells (filled histogram) by Flow Cytometry. Mouse IgG1 ? Isotype Control FITC is shown in the open histogram. Cells in the lymphocyte gate were used for analysis.