Anti-CD8 alpha抗体[53-6.7] (Allophycocyanin/Cy5.5 ® ) (ab82005)

概述

  • 产品名称Anti-CD8 alpha抗体[53-6.7] (Allophycocyanin/Cy5.5 ® )
    参阅全部 CD8 alpha 一抗
  • 描述
    大鼠单克隆抗体[53-6.7] to CD8 alpha (Allophycocyanin/Cy5.5 ® )
  • 偶联物Allophycocyanin/Cy5.5 ® . Ex: 650nm, Em: 690nm
  • 特异性Specific for the a chain of the murine CD8 heterodimer
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, , Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 阳性对照
    • Mouse mesenteric lymph node cells.
  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明In vivo and in vitro treatment with the clone 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. Clone 53-6.7 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
  • 克隆单克隆
  • 克隆编号53-6.7
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Depletion Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Fractionation of CD8a+ T cells.

靶标

  • 功能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 疾病相关Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha polypeptide (p32) antibody
    • CD8 antibody
    • CD8 antigen alpha polypeptide antibody
    • CD8 antigen alpha polypeptide (p32) antibody
    • CD8a antibody
    • CD8A antigen antibody
    • CD8A molecule antibody
    • CD8A_HUMAN antibody
    • Leu2 antibody
    • Leu2 T lymphocyte antigen antibody
    • Ly3 antibody
    • LYT3 antibody
    • MAL antibody
    • OKT8 T cell antigen antibody
    • OTTHUMP00000160760 antibody
    • OTTHUMP00000160764 antibody
    • OTTHUMP00000203528 antibody
    • OTTHUMP00000203721 antibody
    • p32 antibody
    • T cell antigen Leu2 antibody
    • T cell co receptor antibody
    • T-cell surface glycoprotein CD8 alpha chain antibody
    • T-lymphocyte differentiation antigen T8/Leu-2 antibody
    • T8 T cell antigen antibody
    • T8/Leu-2 T-lymphocyte differentiation antigen antibody
    see all

Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin/Cy5.5 ® ) (ab82005)参考文献

This product has been referenced in:
  • Takahashi K  et al. CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A 89:5557-61 (1992). ; Mouse . Read more (PubMed: 1608966) »
  • Ledbetter JA  et al. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med 152:280-95 (1980). Flow Cyt ; Mouse . Read more (PubMed: 6156984) »

See all 3 Publications for this product

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