Anti-CD8 alpha抗体[53-6.7] (Allophycocyanin) (ab25499)

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概述

  • 产品名称Anti-CD8 alpha抗体[53-6.7] (Allophycocyanin)
    参阅全部 CD8 alpha 一抗
  • 描述
    大鼠单克隆抗体[53-6.7] to CD8 alpha (Allophycocyanin)
  • 偶联物Allophycocyanin. Ex: 645nm, Em: 660nm
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, Functional Studiesmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 常规说明

    This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze. Store undiluted.
  • 存储溶液pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride, 0.1% Gelatin
  • Concentration information loading...
  • 纯度Affinity purified
  • Primary antibody说明This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
  • 克隆单克隆
  • 克隆编号53-6.7
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab25499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab129719-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

In vivo and in vitro depletion of CD8α+ cells. Fractionation of CD8α+ T cells.

靶标

  • 功能Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
  • 疾病相关Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻译后修饰All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • alpha polypeptide (p32) antibody
      • CD8 antibody
      • CD8 antigen alpha polypeptide antibody
      • CD8 antigen alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8A antigen antibody
      • CD8A molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • Ly3 antibody
      • LYT3 antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • OTTHUMP00000160760 antibody
      • OTTHUMP00000160764 antibody
      • OTTHUMP00000203528 antibody
      • OTTHUMP00000203721 antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      • T8/Leu-2 T-lymphocyte differentiation antigen antibody
      see all

    Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin) 图像

    • Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin) (ab25499)
      Flow Cytometry - Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin) (ab25499)

      Flow Cytometry analysis of C57Bl/6 splenocytes labeling CD8 alpha with ab25499 at 0.25 µg (solid line), and Rat IgG2a (Allophycocyanin) isotype control at 0.25 µg (dashed line).

    Anti-CD8 alpha antibody [53-6.7] (Allophycocyanin) (ab25499)参考文献

    This product has been referenced in:
    • Anraku M  et al. Synergistic antitumor effects of regulatory T cell blockade combined with pemetrexed in murine malignant mesothelioma. J Immunol 185:956-66 (2010). Flow Cyt ; Mouse . Read more (PubMed: 20548032) »

    See 1 Publication for this product

    发表研究结果有使用 ab25499？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    1 Abreview or Q&A

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    Dear abcam support desk, Thankyou so much for your fast response. I’mwondering if you still sell this item ab52854 because this antibody works great onour mouse section. Ifnot, do you have any recommendation? Let me know. Thank You for your help.

    Thank you for contacting us. We have many other products that works well on mouse tissues and are fully tested. The catalogue numbers are Un conjugated anti CD8 antibodies ab22378 ab25478 ab3081 ab108292 Conjugated anti CD8 antibodies ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"