Anti-CD8 alpha抗体[53-6.7] (ab25478)
Key features and details
- Rat monoclonal [53-6.7] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-CD8 alpha抗体[53-6.7]
参阅全部 CD8 alpha 一抗 -
描述
大鼠单克隆抗体[53-6.7] to CD8 alpha -
宿主
Rat -
特异性
ab25478 recognises a chain of the murine CD8 heterodimer. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to CD8 alpha. Spleen cells or thymocyte membranes
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常规说明
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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纯度
Affinity purified -
Primary antibody说明
This antibody blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. -
克隆
单克隆 -
克隆编号
53-6.7 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- Biotin Anti-CD8 alpha antibody [53-6.7] (ab25176)
- PE/Texas Red® Anti-CD8 alpha antibody [53-6.7] (ab25294)
- PE Anti-CD8 alpha antibody [53-6.7] (ab25498)
- APC Anti-CD8 alpha antibody [53-6.7] (ab25499)
- FITC Anti-CD8 alpha antibody [53-6.7] (ab25676)
- PE Anti-CD8 alpha antibody [53-6.7] (ab272343)
- Cy5 ® Anti-CD8 alpha antibody [53-6.7] (ab39850)
- PE/Cy7® Anti-CD8 alpha antibody [53-6.7] (ab39853)
- APC/Cy5.5® Anti-CD8 alpha antibody [53-6.7] (ab82005)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab25478于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 2µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 2µg for 106 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
疾病相关
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12525 Mouse
- SwissProt: P01731 Mouse
- Unigene: 1858 Mouse
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别名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab25478 被引用在 11 文献中.
- Abu Tayeh A et al. Psoriasis-like skin disorder in transgenic mice expressing a RIG-I Singleton-Merten syndrome variant. Int Immunol 33:211-224 (2021). PubMed: 33119735
- Balam S et al. Cross-presentation of dead-cell-associated antigens by DNGR-1+ dendritic cells contributes to chronic allograft rejection in mice. Eur J Immunol 50:2041-2054 (2020). PubMed: 32640051
- Yoo B et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). PubMed: 30886310
- Ritsma L et al. Integrin ß1 activation induces an anti-melanoma host response. PLoS One 12:e0175300 (2017). IHC, ICC/IF ; Mouse, Human . PubMed: 28448494
- Hao Z et al. K48-linked KLF4 ubiquitination by E3 ligase Mule controls T-cell proliferation and cell cycle progression. Nat Commun 8:14003 (2017). PubMed: 28084302