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The details of the immunogen for this antibody are not available.
In chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha and CD8 beta chains, a relatively large proportion of the CD8+ TCR gamma delta cells in the spleens of embryos and young chicks express only the alpha chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.
Our Abpromise guarantee covers the use of ab24910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
ab24910 can be also used for purification of CD8+ T cells by positive cell sorting.
|Flow Cyt||Use 1µg for 106 cells.
ab18427 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.
|IP||Use at an assay dependent concentration.|
ab24910 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"