Anti-CD79a抗体[HM57] (ab62650)


  • 产品名称Anti-CD79a抗体[HM57]
    参阅全部 CD79a 一抗
  • 描述
    小鼠单克隆抗体[HM57] to CD79a
  • 经测试应用适用于: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Human, Pig, Monkey
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 202-216 of Human CD79a

  • 阳性对照
    • Human tonsil tissue.



Our Abpromise guarantee covers the use of ab62650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: 1/10 - 1/50. Use 10µl to label 1x106 cells in 100µl. Membrane permeabilisation is required for this application.
    IHC-P: 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
    • 组织特异性B-cells.
    • 疾病相关Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
    • 序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • 翻译后修饰Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
    • 细胞定位Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • B lymphocyte-specific MB1 protein antibody
      • B-cell antigen receptor complex-associated protein alpha chain antibody
      • CD 79a antibody
      • CD79a antibody
      • CD79a antigen (immunoglobulin-associated alpha) antibody
      • CD79A antigen antibody
      • CD79a molecule, immunoglobulin-associated alpha antibody
      • CD79A_HUMAN antibody
      • Ig alpha antibody
      • Ig-alpha antibody
      • IGA antibody
      • IgM-alpha antibody
      • Immunoglobulin-associated alpha antibody
      • Ly54 antibody
      • MB-1 membrane glycoprotein antibody
      • MB1 antibody
      • Membrane-bound immunoglobulin-associated protein antibody
      • Surface IgM-associated protein antibody
      see all

    Anti-CD79a antibody [HM57] (ab62650)参考文献

    ab62650 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Ferret Tissue sections (Lymph nodes)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial Target Retrieval Solution Low pH
    Permeabilization No
    Specification Lymph nodes
    Fixative Formaldehyde

    Mrs. Jenni Harper

    Verified customer

    提交于 Sep 19 2016