概述

  • 产品名称Anti-CD77抗体[38-13]
  • 描述
    大鼠单克隆抗体[38-13] to CD77
  • 特异性CD77 is specific for Burkitt's lymphoma associated antigen (BLA), which is a neutral glycosphingolipid of the globoseries: globotriaosylceramide Gb3. Reacts with red blood cells of phenotype pk1 or pk2 and activated B cells in agglutination assay at 4°C and the radioactive Coombs test. Does not react with P or P2 red cells, slightly agglutinate P1 red cells after papain treatment (anti pk activity).
  • 经测试应用适用于: ICC/IF, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Daudi cell line (Burkitt's lymphoma).

性能

应用

Our Abpromise guarantee covers the use of ab19795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/10.

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Anti-CD77 antibody [38-13] 图像

  • Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1X PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98368) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD77 antibody [38-13] (ab19795)参考文献

This product has been referenced in:
  • Jeon YJ  et al. Epithelial-Mesenchymal Transition in Kidney Tubular Epithelial Cells Induced by Globotriaosylsphingosine and Globotriaosylceramide. PLoS One 10:e0136442 (2015). ICC/IF ; Human . Read more (PubMed: 26291612) »
  • Lakomá J  et al. Pain related channels are differentially expressed in neuronal and non-neuronal cells of glabrous skin of fabry knockout male mice. PLoS One 9:e108641 (2014). Read more (PubMed: 25337704) »

See all 3 Publications for this product

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The laboratory that supplies this to us does not check the concentration of the antibody solution, so matching an isotype control concentration to the concentration of the antibody will not be possible, unless yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"