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Tissue/ cell preparation - Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
Our Abpromise guarantee covers the use of ab77676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.|
|Flow Cyt||Use 1µg for 106 cells.
ab18400 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration.|
ab77676 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"