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Tissue/ cell preparation - Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
Our Abpromise guarantee covers the use of ab77676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.|
|Flow Cyt||Use 1µg for 106 cells. ab18400-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
ab77676 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"