Anti-CD74抗体[MRC OX3] (ab22348)


  • 产品名称Anti-CD74抗体[MRC OX3]
    参阅全部 CD74 一抗
  • 描述
    小鼠单克隆抗体[MRC OX3] to CD74
  • 特异性Recognises a polymorphic determinant of rat Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. The antibody cross reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognises Ia antigens on B-cells, dendritic cells and certain epithelial cells. It is particularly useful for distinguishing Ia positive cells from different rat strains, eg for recognising cells of donor origin in bone marrow reconstituted radiation chimeras.
  • 经测试应用适用于: IHC-R, IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Rat thymocyte membrane glycoproteins


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
  • 克隆单克隆
  • 克隆编号MRC OX3
  • 骨髓瘤NS1
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab22348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-R Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • 序列相似性Contains 1 thyroglobulin type-1 domain.
  • 细胞定位Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

Anti-CD74 antibody [MRC OX3] (ab22348)参考文献

ab22348 has not yet been referenced specifically in any publications.

Product Wall

We do not have data that answers your questions but the following paper should contain information about the production and characterization of the OX3 clone. I do not have access to the journal to confirm.

McMaster, W. R. & Williams, A. F. ...

Read More

The antibody does not require antigen retrieval. The rest of the protocol is standard, and can be found on our website at the following link.

For a secondary antibody, ...

Read More

Yes, "IHC-P" refers to immunohistochemistry of formalin-fixed, paraffin-embedded tissue.

Regarding mouse reactivity, it has been confirmed that the antibody reacts with mouse strains (MHC haplotypes b and s).

If you are using a se...

Read More