概述

  • 产品名称Anti-CD73抗体- C-terminal
    参阅全部 CD73 一抗
  • 描述
    兔多克隆抗体to CD73 - C-terminal
  • 经测试应用适用于: ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human CD73 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • Human breast carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab71822 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 25375632

靶标

  • 功能Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 疾病相关Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • 序列相似性Belongs to the 5'-nucleotidase family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody - C-terminal 图像

  • Anti-CD73 antibody - C-terminal (ab71822) at 1/100 dilution + Mouse brain tissure lysate at 35 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 31 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ab71822 at 1/50 dilution, staining CD73 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-CD73 antibody - C-terminal (ab71822)参考文献

This product has been referenced in:
  • Clauser L  et al. Selective augmentation of stem cell populations in structural fat grafts for maxillofacial surgery. PLoS One 9:e110796 (2014). ICC/IF ; Human . Read more (PubMed: 25375632) »
  • Kurth TB  et al. Functional mesenchymal stem cell niches in adult mouse knee joint synovium in vivo. Arthritis Rheum 63:1289-300 (2011). Read more (PubMed: 21538315) »

See all 2 Publications for this product

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