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Our Abpromise guarantee covers the use of ab54217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22164136|
|IP||Use at an assay dependent concentration. Use 10µg per test.|
|IHC-Fr||Use a concentration of 1 - 5 µg/ml.|
|Flow Cyt||Use a concentration of 1 - 2 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration. PubMed: 21612554|
|ICC/IF||Use at an assay dependent concentration. PubMed: 19937263|
|ICC||Use at an assay dependent concentration.|
ab54217 staining cultured human bone marrow stem cells by ICC/IF. Cells were formaldehyde fixed, permeabilization not done prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with sample for 12 hours at 4°C. FITC conjugated rabbit polyclonal to mouse diluted 1/1000 was used as the secondary.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"