概述

  • 产品名称
    Anti-CD73抗体[7G2]
    参阅全部 CD73 一抗
  • 描述
    小鼠单克隆抗体[7G2] to CD73
  • 经测试应用
    适用于: IHC-FoFr, IP, IHC-Fr, Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length purified Human placental CD73.

  • 阳性对照
    • Flow Cyt: Human peripheral blood lymphocytes.

性能

应用

Our Abpromise guarantee covers the use of ab54217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 22164136
IP Use at an assay dependent concentration. Use 10µg per test.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration. PubMed: 21612554
ICC/IF Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
    • 疾病相关
      Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
    • 序列相似性
      Belongs to the 5'-nucleotidase family.
    • 细胞定位
      Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 5' NT antibody
      • 5' nucleotidase (CD73) antibody
      • 5' nucleotidase precursor antibody
      • 5' nucleotidase, ecto antibody
      • 5' nucleotidase, ecto (CD73) antibody
      • 5'-NT antibody
      • 5'-nucleotidase antibody
      • 5NTD_HUMAN antibody
      • CD73 antibody
      • CD73 antigen antibody
      • E5NT antibody
      • Ecto 5' nucleotidase antibody
      • Ecto-5'-nucleotidase antibody
      • eN antibody
      • eNT antibody
      • NT antibody
      • NT5 antibody
      • NT5E antibody
      • NTE antibody
      • Purine 5 Prime Nucleotidase antibody
      see all

    图片

    • ab54217 staining human peripheral blood lymphocytes by FACS analysis.
    • ab54217 staining cultured human bone marrow stem cells by ICC/IF. Cells were formaldehyde fixed, permeabilization not done prior to blocking in 1% BSA for 30 minutes at 22°C. The primary antibody was diluted 1/250 and incubated with sample for 12 hours at 4°C. FITC conjugated rabbit polyclonal to mouse diluted 1/1000 was used as the secondary.

    文献

    This product has been referenced in:
    • Jia Z  et al. Comparison of biological characteristics of nucleus pulposus mesenchymal stem cells derived from non-degenerative and degenerative human nucleus pulposus. Exp Ther Med 13:3574-3580 (2017). Read more (PubMed: 28588682) »
    • Celik S  et al. Extracting a low-dimensional description of multiple gene expression datasets reveals a potential driver for tumor-associated stroma in ovarian cancer. Genome Med 8:66 (2016). Read more (PubMed: 27287041) »

    See all 10 Publications for this product

    客户评价及客户问答

    Merci de nous avoir contactés.

    Malheureusement, nous n'avons pas d’hybridome dans notre catalogue.

    Je vous recommande de regarder sur le site Web Biocompare qui vous permettra de rechercher des d'anticorps et leurs fourni...

    Read More

    Thank you for contacting us.

    We apologize for this error. The concentration should be read as 1-5ug/ml; the datasheet has been updated.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any mo...

    Read More

    Thank you for your reply.

    I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
    (1) Redeemed against the original invoice if this hasn't already been paid.
    (2)...

    Read More

    Thank you for contacting us.

    Apologies for the delay in getting back to you. Unfortunately we do not have another batch of this product available. As I mentioned previously I would be happy to refund the item or issue a free unit of another ...

    Read More

    I am sorry for the delay in responding to your request for another batch of ab54217.

    I have been in contact with the lab and unfortunately there is not another lot available. I would be happy to refund the item or issue a free unit of anothe...

    Read More

    Thank you for responding to our survey.
    I am sorry to hear the replacement vial did not work as expected. I am currently in contact with the lab to identify whether another batch is available. I will be back in touch as soon as I can. If there is ...

    Read More

    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    DISCOUNT CODE: XXXXXXXXXXXXXXX
    Expiration date: 18-08-2012

    I am very pleased to hear you would like to accept our offer and test ab11415 and ab11155in [Sheep]. Th...

    Read More
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Adipose and bone marrow stem cells)
    Specification
    Adipose and bone marrow stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - TRITON x100 0,1%
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Username

    Ms. Vega Villar

    Verified customer

    提交于 Mar 29 2012

    Merci de nous avoir contactés et désolé pour le délai de ma réponse.
    Je viens d'être informé que l'anti-CD73 clone [7G2] (référence ab54217) a été décrit pour des tests d'inhibition dans la publication suivante : Thomson LF, et al. Tissue Antigens...

    Read More

    Thank you for your reply.

    For dilution of the antibodies ready to use in the experiment, as discussed in the previous email I can suggest to dilute antibodiesin PBS orTBS. It is sometimes helpful to include 0.2% Tween 20, however this may ne...

    Read More

    1-10 of 14 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册