Anti-CD73抗体[4G6E3] (ab202122)
Key features and details
- Mouse monoclonal [4G6E3] to CD73
- Suitable for: Flow Cyt, IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD73抗体[4G6E3]
参阅全部 CD73 一抗 -
描述
小鼠单克隆抗体[4G6E3] to CD73 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human CD73 aa 1-250. Expressed in E. Coli.
Database link: P21589 -
阳性对照
- Flow Cytometry: A431 cells; WB: A375, and A431 cell lysates; IHC-P: Human endometrial cancer and Human esophageal cancer tissues.
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常规说明
This product was changed from ascites to supernatant. Lot no’s high than GR287343-13 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
4G6E3 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab202122于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P | (1) |
1/200 - 1/1000.
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WB |
1/500 - 1/2000.
|
说明 |
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Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/200 - 1/1000. |
WB
1/500 - 1/2000. |
靶标
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功能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
疾病相关
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
序列相似性
Belongs to the 5'-nucleotidase family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281363 Cow
- Entrez Gene: 4907 Human
- Entrez Gene: 23959 Mouse
- Entrez Gene: 58813 Rat
- Omim: 129190 Human
- SwissProt: Q05927 Cow
- SwissProt: P21589 Human
- SwissProt: Q61503 Mouse
see all -
别名
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
图片
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All lanes : Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution
Lane 1 : Wild-type A-375 cell lysate
Lane 2 : NT5E knockout A-375 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 65 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CD73 antibody [4G6E3] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab202122 was shown to bind specifically to CD73. A band was observed at 65-70 kDa in wild-type A-375 cell lysates with no signal observed at this size in NT5E knockout cell line ab281591 (knockout cell lysate ab282943). A cross-reactive band is observed at 65 kDa in all samples. To generate this image, wild-type and NT5E knockout A-375 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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Flow cytometric analysis of A431 cells using ab202122 at 1/200 (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded Human esophageal cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.
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All lanes : Anti-CD73 antibody [4G6E3] (ab202122) at 1/1000 dilution
Lane 1 : Wild-type A-431 whole cell lysate
Lane 2 : NT5E knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A375 (Human malignant melanoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.Lanes 1 - 3: Merged signal (red and green). Green - ab202122 observed at 63 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab202122 was shown to recognize NT5E in wild-type A-431 cells as signal was lost at the expected MW in NT5E knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and NT5E knockout samples were subjected to SDS-PAGE. Ab202122 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + A431 cell lysate
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Anti-CD73 antibody [4G6E3] (ab202122) at 1/500 dilution + Human CD73 (AA: 30-250) recombinant protein
Expected MW is 26.6 kDa
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Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling CD73 with ab202122 at 1/200 with DAB staining.
实验方案
数据表及文件
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Datasheet download
文献 (10)
ab202122 被引用在 10 文献中.
- Li X LncRNA metastasis-associated lung adenocarcinoma transcript-1 promotes osteogenic differentiation of bone marrow stem cells and inhibits osteoclastic differentiation of Mø in osteoporosis via the miR-124-3p/IGF2BP1/Wnt/β-catenin axis. J Tissue Eng Regen Med 16:311-329 (2022). PubMed: 34962086
- Xie A et al. Kartogenin Induced Adipose-Derived Stem Cell Exosomes Enhance the Chondrogenic Differentiation Ability of Adipose-Derived Stem Cells. Dis Markers 2022:6943630 (2022). PubMed: 36072901
- Zheng WV et al. Anti-aging effect of β-carotene through regulating the KAT7-P15 signaling axis, inflammation and oxidative stress process. Cell Mol Biol Lett 27:86 (2022). PubMed: 36209059
- Hu M et al. The function and mechanism of the miR-210-3p/KRAS axis in bone marrow-derived mesenchymal stem cell from patients with osteoporosis. J Tissue Eng Regen Med 15:699-711 (2021). PubMed: 33982450
- Ren X et al. Osteogenic ability using porous hydroxyapatite scaffold-based delivery of human placenta-derived mesenchymal stem cells. Exp Ther Med 22:1091 (2021). PubMed: 34504545
- Zhang J et al. A novel circular RNA circ_HN1/miR-628-5p/Ecto-5'-nucleotidase competing endogenous RNA network regulates gastric cancer development. Bioengineered 12:9739-9752 (2021). PubMed: 34637682
- Li B et al. The MSC-Derived Exosomal lncRNA H19 Promotes Wound Healing in Diabetic Foot Ulcers by Upregulating PTEN via MicroRNA-152-3p. Mol Ther Nucleic Acids 19:814-826 (2020). PubMed: 31958697
- Pan C et al. Pretreatment with human urine-derived stem cells protects neurological function in rats following cardiopulmonary resuscitation after cardiac arrest. Exp Ther Med 20:112 (2020). PubMed: 32989390
- Baracho Trindade Hill A et al. Placental scaffolds have the ability to support adipose-derived cells differentiation into osteogenic and chondrogenic lineages. J Tissue Eng Regen Med 14:1661-1672 (2020). PubMed: 32893450
- Yu L et al. Exosomes derived from microRNA-199a-overexpressing mesenchymal stem cells inhibit glioma progression by down-regulating AGAP2. Aging (Albany NY) 11:5300-5318 (2019). PubMed: 31386624