概述

  • 产品名称Anti-CD73抗体[4G4]
    参阅全部 CD73 一抗
  • 描述
    小鼠单克隆抗体[4G4] to CD73
  • 经测试应用适用于: WB, IP, Flow Cyt, IHC-Fr, Functional Studies, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation : human endothelial cell preparation from inflamed synovial stroma from rheumatoid arthritis patients.

  • 阳性对照
    • HUVEC and fresh lymphocytes.
  • 常规说明


    ab81720 causes a reduction in CD73 expression on lymphocytes.

性能

应用

Our Abpromise guarantee covers the use of ab81720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
IP 1/50.
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50. Tissue sections were fixed in acetone.
Functional Studies Use at an assay dependent concentration. Antibody 4G4 functions as an inhibitor of lymphocyte binding to HUVEC. The antibody was functionally tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer. Furthermore the monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes and reduces enzyme activity.
ICC/IF 1/50.

靶标

  • 功能Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 疾病相关Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • 序列相似性Belongs to the 5'-nucleotidase family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody [4G4] 图像

  • ab81720 at 4 µg/ml detecting CD73 in HUVEC by Flow cytometric analysis. Blue histogram represents detection of CD73. Red and black histograms represent cells without antibody and isotype control respectively.
  • ICC/IF image of ab81720 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81720, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CD73 antibody [4G4] (ab81720)参考文献

This product has been referenced in:
  • Aliagas E  et al. High expression of ecto-nucleotidases CD39 and CD73 in human endometrial tumors. Mediators Inflamm 2014:509027 (2014). IHC ; Human . Read more (PubMed: 24707115) »
  • Texidó L  et al. Ecto-nucleotidases activities in the contents of ovarian endometriomas: potential biomarkers of endometriosis. Mediators Inflamm 2014:120673 (2014). WB ; Human . Read more (PubMed: 25276049) »

See all 9 Publications for this product

Product Wall

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality...

Read More

Thank you for contacting us.

Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

However, instead of using PFA as a fixative, I w...

Read More

Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not be...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"