概述

  • 产品名称
    Anti-CD73抗体[1D7]
    参阅全部 CD73 一抗
  • 描述
    小鼠单克隆抗体[1D7] to CD73
  • 经测试应用
    适用于: WB, ELISA, IHC-P, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified recombinant fragment of Human CD73 expressed in E. coli.

  • 阳性对照
    • Human lung cancer, cholangiocarcinorna, lymph node and esophagus tissues.

性能

应用

Our Abpromise guarantee covers the use of ab91086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. PubMed: 23776241

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 23776241

靶标

  • 功能
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • 疾病相关
    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • 序列相似性
    Belongs to the 5'-nucleotidase family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody [1D7] 图像

  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human cholangiocarcinorna tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human lymph node tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human esophagus tissue by Immunohistochemistry using DAB staining.

Anti-CD73 antibody [1D7] (ab91086)参考文献

This product has been referenced in:
  • Moriguchi T  et al. Gata3 Hypomorphic Mutant Mice Rescued with a Yeast Artificial Chromosome Transgene Suffer a Glomerular Mesangial Cell Defect. Mol Cell Biol 36:2272-81 (2016). IHC ; Mouse . Read more (PubMed: 27296697) »
  • Loi S  et al. CD73 promotes anthracycline resistance and poor prognosis in triple negative breast cancer. Proc Natl Acad Sci U S A 110:11091-6 (2013). WB, IHC-Fr, Flow Cyt ; Human . Read more (PubMed: 23776241) »

See all 2 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH8,5
Sample
Human Tissue sections (appendix)
Specification
appendix
Permeabilization
Yes - Washbuffer from Dako with Tween
Fixative
Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Oct 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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