概述

  • 产品名称Anti-CD70抗体[BU69]
    参阅全部 CD70 一抗
  • 描述
    小鼠单克隆抗体[BU69] to CD70
  • 特异性This antibody inhibits T cell proliferation induced by dendritic cells.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, Functional Studies, Inhibition Assay, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Waldenstroms macroglobulineamia cell line WM-1.

性能

应用

Our Abpromise guarantee covers the use of ab77868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
IHC-P 1/100. PubMed: 23776334
ELISA Use at an assay dependent concentration.

靶标

  • 功能Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
  • 序列相似性Belongs to the tumor necrosis factor family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 27L antibody
    • CD 70 antibody
    • CD27 L antibody
    • CD27 LG antibody
    • CD27 ligand antibody
    • CD27-L antibody
    • CD27L antibody
    • CD27LG antibody
    • CD70 antibody
    • CD70 antigen antibody
    • CD70 molecule antibody
    • CD70_HUMAN antibody
    • Ki 24 antigen antibody
    • Ki24 antigen antibody
    • Surface antigen CD70 antibody
    • TNFSF 7 antibody
    • TNFSF7 antibody
    • Tumor necrosis factor (ligand) superfamily, member 7 antibody
    • Tumor necrosis factor ligand superfamily member 7 antibody
    see all

Anti-CD70 antibody [BU69] 图像

  • ab77868 used in Flow Cytometry.
    Red histogram represents cells stained with secondary alone ab8517 at a 1/200 dilution, blue histogram - Formaldehyde fixed SK-RC-45 human renal carcinoma cells stained with ab78868 at a 1/100 dilution. FL1-H - green channel

    See Abreview

  • Immunohistochemical analysis of Human tonsil tissue, staining CD70 with ab77868.

    Tissue was fixed with acetone and blocked with 6% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA in PBS) for 1 hour at 25°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

Anti-CD70 antibody [BU69] (ab77868)参考文献

This product has been referenced in:
  • Liu N  et al. Increased CD70 expression is associated with clinical resistance to cisplatin-based chemotherapy and poor survival in advanced ovarian carcinomas. Onco Targets Ther 6:615-9 (2013). IHC-P ; Human . Read more (PubMed: 23776334) »
  • Polak ME  et al. CD70-CD27 interaction augments CD8+ T-cell activation by human epidermal Langerhans cells. J Invest Dermatol 132:1636-44 (2012). Human . Read more (PubMed: 22377764) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMCs)
Specification PBMCs
Preparation Cell harvesting/tissue preparation method: Ficoll
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
Username

Abcam user community

Verified customer

提交于 Oct 01 2012

Thank you for your inquiry.

I am afraid we have no data or protocols for the functional testing of this antibody. We rely on data provided by scientistsand we have not done functional studies in house with this antibody.The generation of the ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Recombinant protein (purified CD70 ECD)
Specification purified CD70 ECD
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Type Direct
Username

Ms. Tatiana Karakasheva

Verified customer

提交于 Jun 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (SK-RC-45, renal cell carcinoma, human)
Specification SK-RC-45, renal cell carcinoma, human
Preparation Cell harvesting/tissue preparation method: brief trypsinization
Sample buffer: PBS+0.5%BSA
Fixation Formaldehyde
Permeabilization No
Gating Strategy general cell population
Username

Ms. Tatiana Karakasheva

Verified customer

提交于 Jun 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"