The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
Add 100ml of whole blood to a staining tube.
To this tube add 10ml of CD7 (PE)reagent.
Mix gently and incubate 20-30 minutes at 2-8°C.
Add 2ml of BIO LISANTE(ab1159) at room temperature.
Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
Wash cells two timeswith 3ml of PBS.
Resuspend cells in 0.5-1ml of PBS and analyse.
CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.
10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
Molecular F/P ratio: 1.0
Porwit-MacDonald A et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia14:816-25 (2000).
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