Anti-CD68抗体[KP1], prediluted (ab845)


  • 产品名称Anti-CD68抗体[KP1], prediluted
    参阅全部 CD68 一抗
  • 描述
    小鼠单克隆抗体[KP1] to CD68, prediluted
  • 特异性This antibody is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
  • 经测试应用适用于: Flow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Subcellular fraction of human alveolar macrophages.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • 克隆单克隆
  • 克隆编号KP1
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/4.
IHC-P Use at an assay dependent concentration.
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. High temperature antigen unmasking may improve the staining.
IHC-Fr Use at an assay dependent concentration. Acetone fixed.
ICC/IF Use at an assay dependent concentration.


  • 功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 序列相似性Belongs to the LAMP family.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Anti-CD68 antibody [KP1], prediluted 图像

  • Overlay histogram showing THP1 cells stained with ab845 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab845, 1/4 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-CD68 antibody [KP1], prediluted (ab845)参考文献

This product has been referenced in:
  • Minten C  et al. DARC shuttles inflammatory chemokines across the blood-brain barrier during autoimmune central nervous system inflammation. Brain 137:1454-69 (2014). IHC-Fr ; Human . Read more (PubMed: 24625696) »
  • Algars A  et al. Type and location of tumor-infiltrating macrophages and lymphatic vessels predict survival of colorectal cancer patients. Int J Cancer 131:864-73 (2012). Read more (PubMed: 21952788) »

See all 5 Publications for this product

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