概述

  • 产品名称Anti-CD68抗体[KP1]
    参阅全部 CD68 一抗
  • 描述
    小鼠单克隆抗体[KP1] to CD68
  • 经测试应用适用于: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 阳性对照
    • Tonsil

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, 99% Water
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号KP1
  • 骨髓瘤unknown
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 18804859
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Recommended heat mediated antigen retrieval in pressure cooker for 2-5 minutes or steaming of tissue sections for 45-60 minutes followed by cooling for 10 minutes and washing in distilled water.

In case of enzymatic epitope retrieval - digest with Pepsin enzyme for 30-60 seconds at RT.

IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 序列相似性Belongs to the LAMP family.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Anti-CD68 antibody [KP1] 图像

  • ab955 staining CD68 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in antibody diution reagent) for 30 minutes at 20°C. An undiluted HRP-conjugated Rat anti-mouse/rabbit polymer was used as the secondary antibody.

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  • ab955 staining CD68 in Human ulcerated Oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

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  • ab955 staining CD68 in Human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red)

  • ab955 at a 1/2000 dilution staining CD68 from mouse lung tissue sections (new born pups) by immunohistochemistry (frozen sections). The antibody was incubated with the tissue for 24 hours and then detected using an Alexa-Fluor® 488 Goat anti-mouse IgG. The negative control was secondary antibody alone.

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  • ab955 staining CD68 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% horse serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 15 hours at 4°C. An undiluted HRP-conjugated Horse polyclonal was used as the secondary antibody.

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  • ab955 staining CD68 in Rabbit iliac tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in Dako antibody diluent) for 4 hours at 25°C. An undiluted Beta-galactosidase-conjugated rabbit polyclonal was used as the secondary antibody.

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  • Formalin-fixed, paraffin embedded sections of mouse spleen were stained for CD68 with ab at 1/200 dilution in immunohistochemical analysis. Alexa Fluor 647® conjugated Donkey Anti-Mouse was used as secondary antibody at 1/200 dilution.

  • Immunohistochemical analysis of tonsil labelling CD68 with ab955.

  • ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

    See Abreview

Anti-CD68 antibody [KP1] (ab955)参考文献

This product has been referenced in:
  • Kim J  et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease through Regulating the Visceral Adipose-Tissue Function. Int J Mol Sci 18:N/A (2017). IHC-P ; Mouse . Read more (PubMed: 28420164) »
  • Furukawa S  et al. Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. Sci Rep 7:42413 (2017). IHC ; Human . Read more (PubMed: 28205524) »

See all 48 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (liver)
Permeabilization Yes - 0.05% Triton X-100
Specification liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative Paraformaldehyde
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提交于 Jun 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Apr 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% normal buffered formalin
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提交于 Feb 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (heart)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate buffer pH6
Permeabilization No
Specification heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Normal buffered formalin
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提交于 Feb 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization No
Specification spleen
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative 10% Normal buffered formalin
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提交于 Jan 31 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Mitral valve, vascular interstitial cells)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Antigen Decloaker
Permeabilization Yes - 0.2% Triton X-100
Specification Mitral valve, vascular interstitial cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde
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提交于 Aug 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (muscle)
Permeabilization No
Specification muscle
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Formaldehyde
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提交于 Mar 14 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mammary Gland Tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM pH=6.0
Permeabilization Yes - PBS with 3% Triton
Specification Mammary Gland Tissue
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
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提交于 Aug 04 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
Permeabilization No
Specification Spleen
Blocking step No blocking step used for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative 10% Neutral Buffered Formalin
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提交于 Jun 01 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (iliac)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: ctriate
Permeabilization No
Specification iliac
Fixative Formaldehyde
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提交于 Apr 30 2015

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