概述

  • 产品名称Anti-CD68抗体[ED1]
    参阅全部 CD68 一抗
  • 描述
    小鼠单克隆抗体[ED1] to CD68
  • 特异性Ab31630 recognises a single chain glycoprotein of 110kD that is expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, WB, RIA, IHC-FrFl, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Tissue/ cell preparation: Rat spleen cells

  • 常规说明This product should be stored undiluted.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号ED1
  • 骨髓瘤Sp2/0-Ag14
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab31630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Fixation with acetone is recommended.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 37 kDa.
RIA Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25374513
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Alternatively protein digestion with trypsin or pronase prior to IHC protocol could be preformed.

靶标

  • 功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 序列相似性Belongs to the LAMP family.
  • 翻译后修饰N- and O-glycosylated.
  • 细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Anti-CD68 antibody [ED1] 图像

  • Staining of rat peritoneal macrophages withAlexa Fluor®647 conjugated Mouse anti Rat CD68, clone ED1 (ab31630) following permeabilisation with Leucoperm.

  • Immunofluorescence analysis of rat lymph node  labelling CD 68 with ab31630. DAPI was used, staining DNA. A green CD4 antibody was used to counterstain. The image was taken at a low magnification. A is an Image of DAPI & ab31630, B is an image of the CD4 counterstain & DAPI, C is merged showing the CD4 counterstain, DAPI & ab31630.

  • ab31630 staining CD68 in rat liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer pH6. Samples were then blocked with 1% BSA for 10 minutes at 21°C followed by incubation with the primary antibody at a 1/800 dilution, for 2 hours at 21°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/200 dilution.

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  • ab31630 staining CD68 in rat kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate buffer. Samples were then blocked with 10% serum for 1 hour at 4°C followed by incubation with the primary antibody at a 1/200 dilution, for 12 hours at 4°C. A biotin-conjugated goat anti-mouse IgG polyclonal was used as secondary antibody at a 1/100 dilution.

    See Abreview

Anti-CD68 antibody [ED1] (ab31630)参考文献

This product has been referenced in:
  • Ueno K  et al. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts. Sci Rep 6:28538 (2016). IHC-P ; Mouse . Read more (PubMed: 27329845) »
  • Zhang ZH  et al. An integrated lipidomics and metabolomics reveal nephroprotective effect and biochemical mechanism of Rheum officinale in chronic renal failure. Sci Rep 6:22151 (2016). IHC-P ; Rat . Read more (PubMed: 26903149) »

See all 41 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Injured spinal cord)
Antigen retrieval step None
Permeabilization No
Specification Injured spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 14 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Microglia)
Permeabilization Yes - Triton X100
Specification Microglia
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Ray

Verified customer

提交于 Feb 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Permeabilization Yes - 0.1% Triton X-100
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citrate buffer, pH6.0
Sample Rat Tissue sections (Bladder)
Specification Bladder
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 19 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Sample Rat Tissue sections (Intestine)
Specification Intestine
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 07 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Sample Rat Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 24 2013

Thank you for your enquiry regarding ab31630.
Since the sample and the host species of this primary antibody is the same (mouse), unfortunately there is a chance for getting high non-specific background signal.
You may be able to work around ...

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Thank you for your inquiry.

I can confirm that prediction of the 110 kDa is based on literature references as follows:
1. Bao, F. et al. (2004) Early anti-inflammatory treatment reduces lipid peroxidation and protein nitration after spin...

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Thank you for contacting us.

As discussed please send us the purchase order number. We will then check what went wrong with these antibodies.

Thanks!

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numero...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"