概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD68
  • 经测试应用
    适用于: WB, IHC-P, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse CD68 (internal sequence).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. The detection limit for ab125212 is approximately 0.1ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use a concentration of 1 µg/ml.
IHC-FoFr Use at an assay dependent concentration.

靶标

  • 功能
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 组织特异性
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 序列相似性
    Belongs to the LAMP family.
  • 翻译后修饰
    N- and O-glycosylated.
  • 细胞定位
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

图片

  • ab125212 staining CD68 in Mouse spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS + 2% BSA + 10% FCS) for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-CD68 antibody (ab125212)

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : RAW246.7 cell lysate


    Predicted band size : 35 kDa
    Additional bands at : 90-100 kDa (possible glycosylated form).

    Western blot analysis of rat and mouse tissue lysates, labelling CD68 with ab125212

  • Immunohistochemical analysis of murine spleen tissue, staining CD68 with ab125212.

    Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (undiluted) for 16 hours at 4°C. An undiluted HRP-conjugated horse anti-rat polyclonal IgG was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of murine spleen tissue, staining CD68 with ab125212.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 5% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/400 in 5% goat serum) for 18 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

  • ab125212 staining CD68 in Mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in 10% goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Comunanza V  et al. VEGF blockade enhances the antitumor effect of BRAFV600E inhibition. EMBO Mol Med 9:219-237 (2017). IF ; Human . Read more (PubMed: 27974353) »
  • Lee HH  et al. Chemotherapy induces adaptive drug resistance and metastatic potentials via phenotypic CXCR4-expressing cell state transition in ovarian cancer. PLoS One 12:e0171044 (2017). Read more (PubMed: 28196146) »

See all 42 Publications for this product

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Hippocampus)
Permeabilization
Yes - 0.2% Triton in PBS
Specification
Hippocampus
Blocking step
1% BSA + 5% Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Username

Dr. Sergi Bayod

Verified customer

提交于 Sep 08 2017

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 05 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 24 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
10 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Sergi Bayod

Verified customer

提交于 Jan 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization
No
Specification
jejunum
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 27 2017

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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