概述

  • 产品名称Anti-CD64抗体[10.1]
    参阅全部 CD64 一抗
  • 描述
    小鼠单克隆抗体[10.1] to CD64
  • 特异性This antibody blocks Ig binding to FcR1 receptor. It may block ADCC. It is an indicator of inflammation.
  • 经测试应用适用于: ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human, Baboon, Cynomolgus Monkey
  • 免疫原

    Tissue/ cell preparation (Human): Rheumatoid synovial fluid cells and fibronectin purified human monocytes.

  • 阳性对照
    • Myeloid cell lines U937 and HL60.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号10.1
  • 骨髓瘤Sp2/0-Ag14
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab23351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性The high affinity receptor for immunoglobulin G CD64 is encoded by a family of 3 genes that share over 98% of DNA sequence homology: FCGR1A, FCGR1B and FCGR1C. The FCGR1C gene, like FCGR1A, is located on 1q21. The third gene of this family, FCGR1B, was found at 1p12. CD64 plays a pivotal role in the immune response. It is a glycoprotein (MW 72 kD) that is constitutively expressed on human monocytes and macrophages.
  • 细胞定位Cell membrane; Single-pass type I membrane protein.
  • 数据库链接
  • 别名
    • CD 64 antibody
    • CD64 antibody
    • CD64 antigen antibody
    • CD64A antibody
    • CD64b antibody
    • CD64c antibody
    • Fc fragment of IgG high affinity Ia receptor antibody
    • Fc fragment of IgG high affinity Ib receptor antibody
    • Fc fragment of IgG high affinity Ic receptor antibody
    • Fc fragment of IgG, high affinity Ia, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for (CD64) antibody
    • Fc fragment of IgG, high affinity Ia, receptor for antibody
    • Fc fragment of IgG, high affinity Ib, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ib, receptor for antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64) antibody
    • Fc fragment of IgG, high affinity Ic, receptor (CD64), pseudogene antibody
    • Fc fragment of IgG, high affinity Ic, receptor for antibody
    • Fc gamma receptor antibody
    • Fc gamma receptor I A1 antibody
    • Fc gamma receptor I antibody
    • Fc gamma receptor I B2 antibody
    • Fc gamma RI antibody
    • Fc gamma RIA antibody
    • Fc gamma RIB antibody
    • Fc of IgG high affinity Ia receptor antibody
    • Fc-gamma RIC antibody
    • FCG 1 antibody
    • FCG1 antibody
    • FcgammaRI antibody
    • FcgammaRIa antibody
    • FCGR 1 antibody
    • FCGR1 antibody
    • FCGR1A antibody
    • FCGR1B antibody
    • FCGR1C antibody
    • FcRI antibody
    • FcRIB antibody
    • FCRIC antibody
    • FLJ18345 antibody
    • HFcgammaRIB antibody
    • High affinity immunoglobulin gamma Fc receptor I antibody
    • High affinity immunoglobulin gamma Fc receptor IB antibody
    • IGFR 1 antibody
    • IGFR1 antibody
    • IGFRB antibody
    • IGFRC antibody
    • IgG Fc receptor I antibody
    • IgG Fc receptor IB antibody
    • IgG Fc receptor IC antibody
    • Immunoglobulin G Fc receptor I antibody
    • Immunoglobulin G Fc receptor IB antibody
    • Immunoglobulin G Fc receptor IC antibody
    • MGC137713 antibody
    see all

Anti-CD64 antibody [10.1] 图像

  • Overlay histogram showing THP1 cells stained with ab23351(red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab23351, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD64 antibody [10.1] (ab23351)参考文献

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See 1 Publication for this product

Product Wall

Thank you for your reply. I have sent you ab95244 as a replacement for ab23351. As you mentioned ab95244 has not been tested in IHC-P and so we cannot guarantee it will work but it is worth trying. Also I am going to issue you a testing discount for ab...

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It would appear that this antibody has not been tested in IHC-P and that the application was added to the datasheet in error. Please accept my apologies for this mistake and I hope that it did not cause too much trouble. I would be happy to either hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"