概述

  • 产品名称Anti-CD63抗体[TS63]
    参阅全部 CD63 一抗
  • 描述
    小鼠单克隆抗体[TS63] to CD63
  • 特异性Recognises the LIMP antigen, a 30-60 kda (smear) protein.
  • 经测试应用适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Jurkat and HEL cell lines.

  • 阳性对照
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A431. This antibody gave a positive result in IHC in the following FFPE tissue: Human Skin Melanoma.

性能

应用

Our Abpromise guarantee covers the use of ab59479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa. Use under non-reducing conditions.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • 组织特异性Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 细胞定位Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Lysosomal associated membrane protein 3 antibody
    • CD 63 antibody
    • CD63 antibody
    • CD63 antigen (melanoma 1 antigen) antibody
    • CD63 antigen antibody
    • CD63 antigen melanoma 1 antigen antibody
    • CD63 molecule antibody
    • CD63_HUMAN antibody
    • gp55 antibody
    • Granulophysin antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • LAMP3 antibody
    • LIMP antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome associated membrane glycoprotein 3 antibody
    • Mast cell antigen AD1 antibody
    • ME491 antibody
    • Melanoma 1 antigen antibody
    • Melanoma associated antigen ME491 antibody
    • Melanoma associated antigen MLA1 antibody
    • Melanoma-associated antigen ME491 antibody
    • MGC72893 antibody
    • MLA 1 antibody
    • MLA1 antibody
    • NGA antibody
    • Ocular melanoma associated antigen antibody
    • Ocular melanoma-associated antigen antibody
    • OMA81H antibody
    • PTLGP40 antibody
    • Tetraspanin 30 antibody
    • Tetraspanin-30 antibody
    • Tspan 30 antibody
    • Tspan-30 antibody
    • TSPAN30 antibody
    see all

Anti-CD63 antibody [TS63] 图像

  • IHC image of CD63 staining in Human Skin Melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab59479, 5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab59479 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab59479 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HL60 cells stained with ab59479 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab59479, 1 µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight®488 goat anti-Mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-CD63 antibody [TS63] (ab59479)参考文献

This product has been referenced in:
  • Milutinovic B  et al. Glycome complexity of human seminal plasma high molecular mass components: Evaluation of the contribution of acid-soluble glycoproteins/mucins and extracellular vesicles. Arch Biochem Biophys 609:20-30 (2016). Human . Read more (PubMed: 27639309) »
  • Zonneveld MI  et al. Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures. J Extracell Vesicles 3:N/A (2014). WB ; Human . Read more (PubMed: 25206958) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (A549 (non small cell lung carcinoma))
Permeabilization Yes - 0.2% TritonX-100
Specification A549 (non small cell lung carcinoma)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 23 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (kidney fibroblast-like cell line COS-7)
Permeabilization Yes - 0.2% TritonX-100
Specification kidney fibroblast-like cell line COS-7
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 23 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Lewis lung carcinoma)
Permeabilization Yes - 0.2% TritonX-100
Specification Lewis lung carcinoma
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 23 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (human non small cell lung carcinoma A549cells)
Permeabilization Yes - 0.2% Triton X-100
Gating Strategy no gating
Specification human non small cell lung carcinoma A549cells
Preparation Cell harvesting/tissue preparation method: trypsin
Sample buffer: 1x HBSS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 06 2016

Application Flow Cytometry
Sample African Green Monkey Cell (African Green Monkey Fibroblast-like Kidney Cell C)
Permeabilization Yes - 0.2% Triton X-100
Gating Strategy no gating
Specification African Green Monkey Fibroblast-like Kidney Cell C
Preparation Cell harvesting/tissue preparation method: trypsin
Sample buffer: 1x HBSS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (salivary gland)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Permeabilization Yes - Commercial Wash Buffer with Tween
Specification salivary gland
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

提交于 Jun 21 2016

Application Western blot
Sample Human Cell lysate - whole cell (Microglia)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 40 µg
Specification Microglia
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Nicole Fernandes

Verified customer

提交于 Sep 16 2015

Thnak you for contacting Abcam.

In regards to ab8219, I have had the chance to talk with the lab. According to them this antigen is indeed very variable on WB of various material. I am sending the pattern they obtained on non-reducing K562 c...

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Thank you for contacting us.

CD63 antigen is also known as LIMP and LAMP-3. While unmodified protein is about 25kDA, this is heavily glycolylated. AB59476migrates like a smear due to a very complexe N-glycosylation. I would recommend this ...

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