Anti-CD63抗体[MEM-259] (ab8219)

概述

  • 产品名称
    Anti-CD63抗体[MEM-259]
    参阅全部 CD63 一抗
  • 描述
    小鼠单克隆抗体[MEM-259] to CD63
  • 特异性
    CD63 antigen (human)
  • 经测试应用
    适用于: Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). (HPB-ALL T cell line)

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.

性能

应用

Our Abpromise guarantee covers the use of ab8219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 2 µg/ml.

Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • 组织特异性
      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • 序列相似性
      Belongs to the tetraspanin (TM4SF) family.
    • 细胞定位
      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Lysosomal associated membrane protein 3 antibody
      • CD 63 antibody
      • CD63 antibody
      • CD63 antigen (melanoma 1 antigen) antibody
      • CD63 antigen antibody
      • CD63 antigen melanoma 1 antigen antibody
      • CD63 molecule antibody
      • CD63_HUMAN antibody
      • gp55 antibody
      • Granulophysin antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • LAMP3 antibody
      • LIMP antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome associated membrane glycoprotein 3 antibody
      • Mast cell antigen AD1 antibody
      • ME491 antibody
      • Melanoma 1 antigen antibody
      • Melanoma associated antigen ME491 antibody
      • Melanoma associated antigen MLA1 antibody
      • Melanoma-associated antigen ME491 antibody
      • MGC72893 antibody
      • MLA 1 antibody
      • MLA1 antibody
      • NGA antibody
      • Ocular melanoma associated antigen antibody
      • Ocular melanoma-associated antigen antibody
      • OMA81H antibody
      • PTLGP40 antibody
      • Tetraspanin 30 antibody
      • Tetraspanin-30 antibody
      • Tspan 30 antibody
      • Tspan-30 antibody
      • TSPAN30 antibody
      see all

    Anti-CD63 antibody [MEM-259] 图像

    • ab8219 staining CD63 in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody which was used diluted to 10 µg/ml for 1 hour at 20°C. An Alexa Fluor®555-conjugated donkey monoclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

      See Abreview

    • ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.

      See Abreview

    • IHC image of CD63 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8219, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml). 

    Anti-CD63 antibody [MEM-259] (ab8219)参考文献

    This product has been referenced in:
    • Yang Y  et al. Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of a-synuclein via exosome. PLoS One 12:e0180892 (2017). WB ; Rat, Human . Read more (PubMed: 28700687) »
    • Kushner EJ  et al. Excess Centrosomes Perturb Dynamic Endothelial Cell Repolarization During Blood Vessel Formation. Mol Biol Cell N/A:N/A (2016). Read more (PubMed: 27099371) »

    See all 25 Publications for this product

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    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (Blood film)
    Specification
    Blood film
    Permeabilization
    Yes - 0.1M PBS with 1% TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Sep 26 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (fibrosarcoma)
    Specification
    fibrosarcoma
    Preparation
    Cell harvesting/tissue preparation method: Cells lifted off plastic with PBS/EDTA
    Sample buffer: PBS/EDTA
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    live cells
    Username

    Abcam user community

    Verified customer

    提交于 Sep 29 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (Fibrosarcoma HT1080 cell line)
    Specification
    Fibrosarcoma HT1080 cell line
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    no gating
    Username

    Abcam user community

    Verified customer

    提交于 Sep 22 2009

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Fibrosarcoma)
    Specification
    Fibrosarcoma
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    提交于 Aug 25 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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