Anti-CD63抗体[MEM-259] (ab8219)


  • 产品名称Anti-CD63抗体[MEM-259]
    参阅全部 CD63 一抗
  • 描述
    小鼠单克隆抗体[MEM-259] to CD63
  • 特异性CD63 antigen (human)
  • 经测试应用适用于: Flow Cyt, IHC-P, ICC/IFmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). (HPB-ALL T cell line)

  • 阳性对照
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.



Our Abpromise guarantee covers the use of ab8219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 2 µg/ml. Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • 组织特异性Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • 序列相似性Belongs to the tetraspanin (TM4SF) family.
    • 细胞定位Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Lysosomal associated membrane protein 3 antibody
      • CD 63 antibody
      • CD63 antibody
      • CD63 antigen (melanoma 1 antigen) antibody
      • CD63 antigen antibody
      • CD63 antigen melanoma 1 antigen antibody
      • CD63 molecule antibody
      • CD63_HUMAN antibody
      • gp55 antibody
      • Granulophysin antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • LAMP3 antibody
      • LIMP antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome associated membrane glycoprotein 3 antibody
      • Mast cell antigen AD1 antibody
      • ME491 antibody
      • Melanoma 1 antigen antibody
      • Melanoma associated antigen ME491 antibody
      • Melanoma associated antigen MLA1 antibody
      • Melanoma-associated antigen ME491 antibody
      • MGC72893 antibody
      • MLA 1 antibody
      • MLA1 antibody
      • NGA antibody
      • Ocular melanoma associated antigen antibody
      • Ocular melanoma-associated antigen antibody
      • OMA81H antibody
      • PTLGP40 antibody
      • Tetraspanin 30 antibody
      • Tetraspanin-30 antibody
      • Tspan 30 antibody
      • Tspan-30 antibody
      • TSPAN30 antibody
      see all

    Anti-CD63 antibody [MEM-259] 图像

    • ab8219 staining CD63 in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody which was used diluted to 10 µg/ml for 1 hour at 20°C. An Alexa Fluor®555-conjugated donkey monoclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

      See Abreview

    • ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.

      See Abreview

    • IHC image of CD63 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8219, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-CD63 antibody [MEM-259] (ab8219)参考文献

    This product has been referenced in:
    • Kushner EJ  et al. Excess Centrosomes Perturb Dynamic Endothelial Cell Repolarization During Blood Vessel Formation. Mol Biol Cell N/A:N/A (2016). Read more (PubMed: 27099371) »
    • Ghossoub R  et al. Syntenin-ALIX exosome biogenesis and budding into multivesicular bodies are controlled by ARF6 and PLD2. Nat Commun 5:3477 (2014). Read more (PubMed: 24637612) »

    See all 18 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample Human Cell (Blood film)
    Specification Blood film
    Permeabilization Yes - 0.1M PBS with 1% TritonX100
    Fixative Paraformaldehyde

    Dr. Ruma Raha-Chowdhury

    Verified customer

    提交于 Sep 26 2014

    ab2215 should be used at a concentration of 2-4 ug/ml in blocking buffer when incubating your membrane whereas ab8216 should be used at a concentration of 2 ug/ml.

    The gel should be run in non-reducing conditions as these antibodies do not re...

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    We have not tested the stability of ab8219 under the alkaline conditions that you described; thus, we cannot guarantee that it will work under these conditions. The laboratory thinks it is possible that it could work, but only performing the exper...

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    We are not aware of a reference describing production and characterization of this CD63 antibody. The epitope the antibody recognizes on CD63 has not, to our knowledge, been mapped, but we do know that the epitope is extracellular when CD63 is integrat...

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    Thank you for contacting us.

    The antibodies have not been tested for reactivity with bovine samples, and we have not received reports of bovine reactivity from our customers.

    Please do not hesitate to contact us if you need any mo...

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    Thank you for your inquiry.

    Ab8219 (anti-CD63) is supplied as 100ul at a concentration of 1mg/ml. We have a customer who reported successfully using the antibody at a 1/200 dilution (0.2 mg/ml), but it may be necessary to optimize this for you...

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    Thank you for your enquiry.

    I can confirm that the epitope for ab8219 CD63 antibody [MEM-259] has been mapped to the extracellular region of the CD63 protein.

    I hope this will be helpful to you. If you have any further questions, ...

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    Thnak you for contacting Abcam.

    In regards to ab8219, I have had the chance to talk with the lab. According to them this antigen is indeed very variable on WB of various material. I am sending the pattern they obtained on non-reducing K562 c...

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    Thank you for contacting us.

    CD63 antigen is also known as LIMP and LAMP-3. While unmodified protein is about 25kDA, this is heavily glycolylated. AB59476migrates like a smear due to a very complexe N-glycosylation. I would recommend this ...

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    The antibody was validated for western blotting using these conditions:

    Sample: K562 cells (24 millions, lysed with 1% laurylmaltoside in 700 ul), non-reducing SDS, denatured by heat for 5 min.
    Blocking: 5% milk, 4oC overnight

    Read More