概述

  • 产品名称Anti-CD63抗体
    参阅全部 CD63 一抗
  • 描述
    兔多克隆抗体to CD63
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human CD63.

    (Peptide available as ab93074.)

  • 阳性对照
    • This antibody gave a positive signal in Human platelet and HepG2 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab68418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24,43 kDa (predicted molecular weight: 25 kDa).

靶标

  • 功能This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • 组织特异性Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 细胞定位Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Lysosomal associated membrane protein 3 antibody
    • CD 63 antibody
    • CD63 antibody
    • CD63 antigen (melanoma 1 antigen) antibody
    • CD63 antigen antibody
    • CD63 antigen melanoma 1 antigen antibody
    • CD63 molecule antibody
    • CD63_HUMAN antibody
    • gp55 antibody
    • Granulophysin antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • LAMP3 antibody
    • LIMP antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome associated membrane glycoprotein 3 antibody
    • Mast cell antigen AD1 antibody
    • ME491 antibody
    • Melanoma 1 antigen antibody
    • Melanoma associated antigen ME491 antibody
    • Melanoma associated antigen MLA1 antibody
    • Melanoma-associated antigen ME491 antibody
    • MGC72893 antibody
    • MLA 1 antibody
    • MLA1 antibody
    • NGA antibody
    • Ocular melanoma associated antigen antibody
    • Ocular melanoma-associated antigen antibody
    • OMA81H antibody
    • PTLGP40 antibody
    • Tetraspanin 30 antibody
    • Tetraspanin-30 antibody
    • Tspan 30 antibody
    • Tspan-30 antibody
    • TSPAN30 antibody
    see all

Anti-CD63 antibody 图像

  • Anti-CD63 antibody (ab68418) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab68418 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    CD63 has a predicted molecular weight of 25 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This is consistent with the literature which describes a band between 30-60 kDa in human platelets(PMID:1993697).

  • Anti-CD63 antibody (ab68418) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 145 kDa,42 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    The 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to human CD63 antigen.

Anti-CD63 antibody (ab68418)参考文献

This product has been referenced in:
  • Rosas LE  et al. In vitro immunotoxicity assessment of culture-derived extracellular vesicles in human monocytes. J Immunotoxicol 13:652-65 (2016). Read more (PubMed: 27075513) »

See 1 Publication for this product

Product Wall

The one CD63 antibody currently in our catalogue that has been shown to be reactive with rat is ab108950, the mouse clone AD1.

http://www.abcam.com/index.html?datasheet=108950

It is also available as a FITC conjugate, ab108949. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Serum (Purified microvesicles)
Loading amount 1 µg
Specification Purified microvesicles
Gel Running Conditions Reduced Denaturing (12.5% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Abcam user community

Verified customer

提交于 Mar 12 2012

Thank you for your inquiry. Unfortunately, we do not have ab68418 conjugated to biotin. However, I would like to recommend some conjugation kits that we have in our catalog : www.abcam.com/easylink I hope this information is nevertheless helpful ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"