Anti-CD62P抗体[Psel.KO.2.7] (ab54427)

概述

  • 产品名称Anti-CD62P抗体[Psel.KO.2.7]
    参阅全部 CD62P 一抗
  • 描述
    小鼠单克隆抗体[Psel.KO.2.7] to CD62P
  • 特异性This antibody recognizes the CD62P cell surface antigen
  • 经测试应用适用于: IP, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Sheep, Goat, Horse, Cow, Cat, Human
  • 免疫原

    CD62P transfected 300.19 cells (Human)

  • 阳性对照
    • Thrombin activated human platelets
  • 常规说明Shelf Life - 18 months, product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab54427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP
IHC-Fr
Flow Cyt
  • 应用说明Flow Cyt: 1/25 - 1/50. Use 10µl of the suggested working dilution to label 106 cells in 100µl
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
    • 组织特异性Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
    • 疾病相关Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    • 序列相似性Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 9 Sushi (CCP/SCR) domains.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    Anti-CD62P antibody [Psel.KO.2.7] 图像

    • ab54427, at a 1/25 dilution, staining thrombin activated human platelets

    Anti-CD62P antibody [Psel.KO.2.7] (ab54427)参考文献

    This product has been referenced in:
    • Baron DM  et al. Adverse effects of hemorrhagic shock resuscitation with stored blood are ameliorated by inhaled nitric oxide in lambs*. Crit Care Med 41:2492-501 (2013). Sheep . Read more (PubMed: 23887236) »

    See 1 Publication for this product

    Product Wall

    Thank you for your reply.

    I am sorry that you have been having issues with some of our antibodies in Flow Cytometry. Under our Abpromise we guarantee that ab108949, ab54427 & ab95780 should work in flow cytometry on rat samples. If we can...

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    Thank you for your reply.

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    Sorry to keep asking you questions, but the customer ID you gave me is for a Dr Alejandro Gomez, is that correct. I ask as its does not mentioned Bone Interface Group in their address that we have on file and I just ...

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    The antibodies you selected look good to me. To generate a quote we do need to have a shipping address and in your last email, you mentioned that you were at the University of Toronto at the moment. Could you please ...

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    Thank you for your inquiry. I am happy to confirm that we offer an antibody against P selectin (CD62P) that is tested and guaranteed for horse samples (the prize can be found on the top of the datasheet): ab54427 (Anti-CD62P antibody [Psel.KO.2.7...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"