The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明Flow Cyt: Use 0.06 - 0.125 µg for 105 to 108 cells in a final volume of 100 µl.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
组织特异性Expressed in B cell lines and T lymphocytes.
序列相似性Belongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.
Siegelman MH et al. The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell61:611-22 (1990).
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