Anti-CD62L抗体[MEL-14] (Allophycocyanin) (ab41459)

概述

  • 产品名称Anti-CD62L抗体[MEL-14] (Allophycocyanin)
    参阅全部 CD62L 一抗
  • 描述
    大鼠单克隆抗体[MEL-14] to CD62L (Allophycocyanin)
  • 偶联物Allophycocyanin. Ex: 645nm, Em: 660nm
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, Blocking, Microcytotoxicity testingmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    C3H/eb cloned mouse B lymphoma 38C-13

  • 常规说明


    The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.

性能

应用

Our Abpromise guarantee covers the use of ab41459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.1µg for 106 cells.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.

In vivo and in vitro blocking of adhesion.

Microcytotoxicity testing Use at an assay dependent concentration.

靶标

  • 功能Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • 组织特异性Expressed in B cell lines and T lymphocytes.
  • 序列相似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Anti-CD62L antibody [MEL-14] (Allophycocyanin) (ab41459)参考文献

This product has been referenced in:
  • Ley K  et al. Sequential contribution of L- and P-selectin to leukocyte rolling in vivo. J Exp Med 181:669-75 (1995). Read more (PubMed: 7530761) »
  • Siegelman MH  et al. The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell 61:611-22 (1990). Read more (PubMed: 1693096) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (splenocyte)
Specification splenocyte
Preparation Cell harvesting/tissue preparation method: cell suspension
Sample buffer: PBS
Permeabilization No
Gating Strategy Live cells
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提交于 May 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"