重组Anti-CD62L抗体[MEL-14] (ab119834)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [MEL-14] to CD62L
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-CD62L抗体[MEL-14]
参阅全部 CD62L 一抗 -
描述
大鼠单克隆抗体[MEL-14] to CD62L -
宿主
Rat -
特异性
ab119834 reacts with mouse CD62L, a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells. -
经测试应用
适用于: Flow Cyt, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD62L. C3H/eb Mouse B cell lymphoma 38C-13.
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阳性对照
- ICC/IF: Mouse spleen cell. Flow Cyt: Mouse splenocytes.
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常规说明
This product was switched from a hybridoma to a recombinant production format on 25th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
克隆
单克隆 -
克隆编号
MEL-14 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119834于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/1000.
|
|
ICC/IF |
1/100.
|
说明 |
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Flow Cyt
1/1000. |
ICC/IF
1/100. |
靶标
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功能
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
组织特异性
Expressed in B cell lines and T lymphocytes. -
序列相似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 20343 Mouse
- SwissProt: P18337 Mouse
- Unigene: 1461 Mouse
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别名
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
图片
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spleen cells labelling CD62L with ab119834 at 1/100 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subset of mouse spleen cells is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab119834 at 1/100 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
-ve control 2: ab179513 at 1/200 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow cytometric analysis of Mouse splenocyte cells labelling CD62L with ab119834 at 1/500 dilution (0.2 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG (Left) or ab119834 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor®647 and anti-CD3 conjugated to PerCP.
Gated on viable CD3+ T cells. -
Flow cytometric analysis of Mouse splenocyte cells labelling CD62L with ab119834 at 1/500 dilution (0.2 ug). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rat IgG (Left) or ab119834 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor®647.Gated on viable lymphocytes.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab119834 被引用在 8 文献中.
- Fischer A et al. Neutrophils direct preexisting matrix to initiate repair in damaged tissues. Nat Immunol 23:518-531 (2022). PubMed: 35354953
- Ye H et al. Nanosponges of circulating tumor-derived exosomes for breast cancer metastasis inhibition. Biomaterials 242:119932 (2020). PubMed: 32169772
- Linterman MA et al. CD28 expression is required after T cell priming for helper T cell responses and protective immunity to infection. Elife 3:N/A (2014). PubMed: 25347065
- Gallatin WM et al. A cell-surface molecule involved in organ-specific homing of lymphocytes. 1983. J Immunol 177:5-9 (2006). PubMed: 16785489
- Chen BJ et al. Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease. Blood 103:1534-41 (2004). PubMed: 14551132
- Friedline RH et al. L-selectin is not required for T cell-mediated autoimmune diabetes. J Immunol 168:2659-66 (2002). PubMed: 11884430
- Mannering SI & Cheers C Interleukin-2 and loss of immunity in experimental Mycobacterium avium infection. Infect Immun 70:27-35 (2002). PubMed: 11748160
- Brawand P et al. Hierarchal utilization of different T-cell receptor Vbeta gene segments in the CD8(+)-T-cell response to an immunodominant Moloney leukemia virus-encoded epitope in vivo. J Virol 73:9161-9 (1999). Flow Cyt ; Mouse . PubMed: 10516023