概述

  • 产品名称Anti-CD62E抗体
    参阅全部 CD62E 一抗
  • 描述
    兔多克隆抗体to CD62E
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide surrounding aa 584 of human CD62E

  • 阳性对照
    • Jurkat cell lysate, mouse small intestine and rat kidney tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab18981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 67 kDa.Can be blocked with CD62E peptide (ab113900).

靶标

  • 功能Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
  • 序列相似性Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 6 Sushi (CCP/SCR) domains.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD26E antibody
    • CD62 antigen-like family member E antibody
    • CD62E antibody
    • E selectin antibody
    • E-selectin antibody
    • ELAM antibody
    • ELAM-1 antibody
    • ELAM1 antibody
    • Endothelial adhesion molecule 1 antibody
    • Endothelial leukocyte adhesion molecule 1 antibody
    • ESEL antibody
    • LECAM2 antibody
    • Leukocyte-endothelial cell adhesion molecule 2 antibody
    • LYAM2_HUMAN antibody
    • Sele antibody
    • Selectin E antibody
    see all

Anti-CD62E antibody 图像

  • ab18981 staining CD62E in Rat brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/50 in 1% BSA) for 18 hours at 4°C. A donkey anti-rabbit DyLight® 650 (IgG; 1/500) was used as the secondary antibody.

    See Abreview

  • Anti-CD62E antibody (ab18981) at 0.5 µg/ml + Jurkat

    Predicted band size : 67 kDa

    Secondary antibody - goat anti-rabbit HRP (ab6721)

  • Anti-CD62E antibody (ab18981) at 2 µg/ml + SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 67 kDa
    Observed band size : 67 kDa

Anti-CD62E antibody (ab18981)参考文献

This product has been referenced in:
  • Wu F  et al. CXCR2 is essential for cerebral endothelial activation and leukocyte recruitment during neuroinflammation. J Neuroinflammation 12:98 (2015). WB ; Mouse . Read more (PubMed: 25990934) »
  • Bopp A  et al. Rac1 promotes diethylnitrosamine (DEN)-induced formation of liver tumors. Carcinogenesis 36:378-89 (2015). Read more (PubMed: 25556150) »

See all 9 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293-T)
Permeabilization Yes - .1% triton
Specification 293-T
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Paraformaldehyde
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提交于 Nov 30 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Rat Cell (Rat Brain Microvascular Endothelial Cells (RBMVEC))
Specification Rat Brain Microvascular Endothelial Cells (RBMVEC)
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 19 2013

1. Deparaffinize/hydrate sections:
a) Incubate sections in three washes of xylene for 5 minutes each.
b) Incubate sections in two washes of 100% ethanol for 10 minutes each.
c) Incubate sections in two washes of 95% ethanol for 10 minute...

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Thank you for your enquiry.

I can confirm that ab18414 can be stored at 4oC as described on the datasheet. This should not be aliquotted and frozen.

The others I would recommend to aliquot and freeze immediately. Keeping them at 4...

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I am very pleased to hear you would like to accept our offer and test ab54426 in IHC-P.

DISCOUNT CODE: ######

Expiration date: ######

This code will give you: 1 free primary antibody before the expiration date. To redee...

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Thank you for contacting us.
I am sorry to hear that the antibody does not seem to work as expected.

As we had discussed, I would suggest to try the following tips to reduce the background as well as shift the MW to a higher band:
<...

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I agree with Heather that it would be useful to have a negative control sample. I may be mistaken but if E-selectin is only expressed exclusively in endothelial cells, perhaps using an extract from a different cell type as a negative control ...

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Thank you for your phone call. I just wanted to follow up with you and send you the suggestions we had discussed over the phone: 1) use peptide in 10-fold excess 2) incubate peptide with Ab for 1-2 h at RT before using for WB 3) avoid prepa...

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