Anti-CD62E抗体[1.2B6] (ab6630)

概述

  • 产品名称
    Anti-CD62E抗体[1.2B6]
    参阅全部 CD62E 一抗
  • 描述
    小鼠单克隆抗体[1.2B6] to CD62E
  • 特异性
    This antibody recognises the human CD62E and CD62P cell surface antigens binding to a common epitope on these members of the selectin family.
  • 经测试应用
    适用于: ELISA, Flow Cyt, IHC-Fr, IP, WBmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    Human E-Selectin (ELAM-1)

  • 常规说明


    This antibody inhibits neutrophil adhesion to E-selectin and to P-selectin. E-selectin (ELAM-1) is expressed on the surface of activated endothelial cells.

性能

应用

Our Abpromise guarantee covers the use of ab6630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 1 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Non-reduced only.
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.
    • 序列相似性
      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD26E antibody
      • CD62 antigen-like family member E antibody
      • CD62E antibody
      • E selectin antibody
      • E-selectin antibody
      • ELAM antibody
      • ELAM-1 antibody
      • ELAM1 antibody
      • Endothelial adhesion molecule 1 antibody
      • Endothelial leukocyte adhesion molecule 1 antibody
      • ESEL antibody
      • LECAM2 antibody
      • Leukocyte-endothelial cell adhesion molecule 2 antibody
      • LYAM2_HUMAN antibody
      • Sele antibody
      • Selectin E antibody
      see all

    图片

    • ab6630 staining CD62E in thrombin activated human peripheral blood platelets by Flow Cytometry analysis.

    文献

    This product has been referenced in:
    • Allen CL  et al. Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing. PLoS One 8:e78441 (2013). Read more (PubMed: 24205233) »
    • Zhou H  et al. NAD(P)H:quinone oxidoreductase 1-compromised human bone marrow endothelial cells exhibit decreased adhesion molecule expression and CD34+ hematopoietic cell adhesion. J Pharmacol Exp Ther 334:260-8 (2010). WB ; Human . Read more (PubMed: 20378716) »

    See all 8 Publications for this product

    客户评价及客户问答

    Thank you for contacting us.

    Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

    However, instead of using PFA as a fixative, I w...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (human nasal tissue)
    Loading amount
    90 µg
    Specification
    human nasal tissue
    Gel Running Conditions
    Non-reduced Denaturing (10 %)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Michael Könnecke

    Verified customer

    提交于 Jan 11 2013

    Thank you for contacting us.

    You are quite correct. As CD62E is a single pass membrane bound protein, there should be no need to permeabilze the cells for an immunocytochemical stain.

    I hope this information is helpful to you....

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Sample
    Human Cell (HUVEC)
    Specification
    HUVEC
    Type
    Direct
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    提交于 Jun 07 2011

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Intestine)
    Specification
    Intestine
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Citrate Buffer
    Username

    Abcam user community

    Verified customer

    提交于 Aug 20 2007

    I have received feedback from the source of ab6630 and ab19264 and would like to share their feedback. Unfortunately I am still awaiting feedback regarding ab2213. For ab6630 we would suggest a working dilution of 10ug/ml. For ab19264 we recommend...

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    The antibody will recognise the native form of the epitope thus Westerns must be conducted in non-reducing conditions (i.e. without DTT/ beta-mercaptoethanol

    The antibody has been characterised extensively and published in: J Immunol 1990 Apr 1;144(7):2558-65 A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide. Well...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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