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Thymocytes and T lymphocytes.
When originally tested in WB, SDS was included in the sample buffer, however, feedback from one researcher has shown that it is best to omit SDS from the sample buffer.
Our Abpromise guarantee covers the use of ab9182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Use under non-reducing conditions.|
|IP||Use at an assay dependent concentration.|
|Flow Cyt||Use a concentration of 1 - 2 µg/ml.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use at an assay dependent concentration. PubMed: 17911601|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Human fibrosarcoma cells stained for CD59 (red) using ab9182 at 10 µg/ml in ICC/IF, followed by Donkey anti-Mouse Alexa 555 at 1/500 dilution.
ab9182 staining CD59 in Human fibrosarcoma (HT1080) by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody,10 µg/ml in PBS, for 1 hour at 20°C. An Abcam PE-conjugated donkey polyclonal to mouse IgG ( ab7003), 5 µg/ml, was used as secondary antibody.
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