Anti-CD55抗体[MEM-118], prediluted (FITC) (ab28112)

概述

  • 产品名称Anti-CD55抗体[MEM-118], prediluted (FITC)
    参阅全部 CD55 一抗
  • 描述
    小鼠单克隆抗体[MEM-118] to CD55, prediluted (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性ab28112 recognizes an epitope in SCR4 domain of CD55 antigen (Decay accelerating factor).
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    T cell line HPB-ALL

  • 常规说明

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

性能

  • 形式Prediluted
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.097% Sodium azide
    Constituents: Tris buffered saline, 0.2% BSA
  • Concentration information loading...
  • 纯度Purified IgM
  • 克隆单克隆
  • 克隆编号MEM-118
  • 同种型IgM
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab28112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 10µl for 106 cells. ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 功能This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
  • 组织特异性Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
  • 序列相似性Belongs to the receptors of complement activation (RCA) family.
    Contains 4 Sushi (CCP/SCR) domains.
  • 结构域The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
  • 翻译后修饰The Ser/Thr-rich domain is heavily O-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 55 antibody
    • CD55 antibody
    • CD55 antigen antibody
    • CD55 Cromer blood group system antibody
    • CD55 molecule antibody
    • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
    • Cd55a antibody
    • Complement decay accelerating factor antibody
    • Complement decay-accelerating factor antibody
    • Complement decay-accelerating factor, GPI-anchored antibody
    • CR antibody
    • CROM antibody
    • Cromer Blood Group antigen antibody
    • Cromer blood group system antibody
    • DAF antibody
    • Daf-GPI antibody
    • DAF_HUMAN antibody
    • Daf1 antibody
    • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
    • Decay accelarating factor 1, isoform CRA_a antibody
    • Decay accelerating factor (GPI-form) antibody
    • Decay Accelerating Factor for Complement antibody
    • Decay accelerating factor GPI-form antibody
    • Decay accelerating factor soluble-form antibody
    • GPI-DAF antibody
    • TC antibody
    see all

Anti-CD55 antibody [MEM-118], prediluted (FITC) 图像

  • Overlay histogram showing THP1 cells stained with ab28112 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28112, 10µl/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM (FITC) (ab91546, 10µl/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD55 antibody [MEM-118], prediluted (FITC) (ab28112)参考文献

ab28112 has not yet been referenced specifically in any publications.

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