Anti-CD55抗体[143-30] (FITC) (ab25634)


  • 产品名称Anti-CD55抗体[143-30] (FITC)
    参阅全部 CD55 一抗
  • 描述
    小鼠单克隆抗体[143-30] to CD55 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性ab25634 recognises CD55, also known as decay accelerating factor (DAF).
  • 经测试应用适用于: Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Human, Pig
  • 免疫原

    The details of the immunogen for this antibody are not available.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号143-30
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab25634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 5µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.


  • 功能This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.
  • 组织特异性Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
  • 序列相似性Belongs to the receptors of complement activation (RCA) family.
    Contains 4 Sushi (CCP/SCR) domains.
  • 结构域The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3.
  • 翻译后修饰The Ser/Thr-rich domain is heavily O-glycosylated.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 55 antibody
    • CD55 antibody
    • CD55 antigen antibody
    • CD55 Cromer blood group system antibody
    • CD55 molecule antibody
    • CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody
    • Cd55a antibody
    • Complement decay accelerating factor antibody
    • Complement decay-accelerating factor antibody
    • Complement decay-accelerating factor, GPI-anchored antibody
    • CR antibody
    • CROM antibody
    • Cromer Blood Group antigen antibody
    • Cromer blood group system antibody
    • DAF antibody
    • Daf-GPI antibody
    • DAF_HUMAN antibody
    • Daf1 antibody
    • Dcay accelerating factor for complement (CD55, Cromer blood group system) antibody
    • Decay accelarating factor 1, isoform CRA_a antibody
    • Decay accelerating factor (GPI-form) antibody
    • Decay Accelerating Factor for Complement antibody
    • Decay accelerating factor GPI-form antibody
    • Decay accelerating factor soluble-form antibody
    • GPI-DAF antibody
    • TC antibody
    see all

Anti-CD55 antibody [143-30] (FITC) 图像

  • Overlay histogram showing peripheral blood lymphocytes stained with ab25634 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25634, 0.5 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.

Anti-CD55 antibody [143-30] (FITC) (ab25634)参考文献

This product has been referenced in:
  • Hofmann A  et al. Proteomic cell surface phenotyping of differentiating acute myeloid leukemia cells. Blood 116:e26-34 (2010). Flow Cyt ; Human . Read more (PubMed: 20570859) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Pig Cell (fetal fibroblast transfected human CD55 gene)
Specification fetal fibroblast transfected human CD55 gene
Preparation Cell harvesting/tissue preparation method: Trypsin/EDTA
Sample buffer: PBS
Fixation Formaldehyde
Permeabilization No
Gating Strategy CD55 expressing cells on the cell surface

Abcam user community

Verified customer

提交于 May 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Blood cells)
Specification Blood cells
Preparation Cell harvesting/tissue preparation method: Trypsin
Sample buffer: PBS
Fixation Formaldehyde
Permeabilization No
Gating Strategy 10000

Ms. kim soohyun

Verified customer

提交于 Apr 27 2009