CD55 293T转染细胞裂解液- (positive control) (ab94035)

概述

  • 产品名称CD55 293T转染细胞裂解液- (positive control)
  • 常规说明ab94035 is a 293T cell transfected lysate in which Human CD55 has been transiently over-expressed using a pCMV-CD55 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • 存储溶液pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 纯度Whole Cell Lysate
  • 研究领域
  • 背景Function: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Tissue specificity: Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix. Similarity: Belongs to the receptors of complement activation (RCA) family. Contains 4 Sushi (CCP/SCR) domains. Domain: The first Sushi domain (SCR1) is not necessary for function. SCR2 and SCR4 provide the proper conformation for the active site on SCR3. PTM: The Ser/Thr-rich domain is heavily O-glycosylated.

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应用

Our Abpromise guarantee covers the use of ab94035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

CD55 293T Transfected Lysate - (positive control) 图像

  • ab94035 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-CD55 antibody (ab54595) at 1/500 dilution

    Lane 1 : CD55 293T Transfected Lysate - (positive control) (ab94035)
    Lane 2 : 293T non-Transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

CD55 293T Transfected Lysate - (positive control) (ab94035)参考文献

ab94035 has not yet been referenced specifically in any publications.

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