概述

  • 产品名称Anti-CD53抗体[MRC OX-44]
    参阅全部 CD53 一抗
  • 描述
    小鼠单克隆抗体[MRC OX-44] to CD53
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IHC-P, IPmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Tissue/ cell preparation (Rat).Rat T cell blasts

  • 表位Clone OX-44 recognises a conformational epitope that is dependent on disulphide bonding.
  • 常规说明


    The antibody recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labelled dendritic cells, granulocytes and microglial cells in the brain.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • Primary antibody说明The antibody recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labelled dendritic cells, granulocytes and microglial cells in the brain.
  • 克隆单克隆
  • 克隆编号MRC OX-44
  • 骨髓瘤NS0
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab22383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/100 - 1/200.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.





ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

ab22383 requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

IP Use at an assay dependent concentration.

靶标

  • 功能May be involved in growth regulation in hematopoietic cells.
  • 组织特异性B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI323659 antibody
    • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
    • CD 53 antibody
    • CD53 antibody
    • CD53 antigen antibody
    • CD53 antigen, isoform CRA_b antibody
    • CD53 glycoprotein antibody
    • Cd53 molecule antibody
    • CD53 tetraspan antigen antibody
    • CD53_HUMAN antibody
    • Cell surface glycoprotein CD53 antibody
    • Leukocyte antigen MRC OX-44 antibody
    • Leukocyte surface antigen CD53 antibody
    • MOX 44 antibody
    • MOX44 antibody
    • OTTHUMP00000059505 antibody
    • Ox-44 antibody
    • OX44 antibody
    • RATOX44 antibody
    • Tetraspanin 25 antibody
    • Tetraspanin-25 antibody
    • Tetraspanin25 antibody
    • Transmembrane glycoprotein antibody
    • TSPAN 25 antibody
    • Tspan-25 antibody
    • TSPAN25 antibody
    see all

Anti-CD53 antibody [MRC OX-44] (ab22383)参考文献

This product has been referenced in:
  • Tomlinson MG  et al. Epitope mapping of anti-rat CD53 monoclonal antibodies. Implications for the membrane orientation of the Transmembrane 4 Superfamily. Eur J Immunol 23:136-40 (1993). Read more (PubMed: 7678222) »
  • Paterson DJ  et al. The MRC OX-44 antigen marks a functionally relevant subset among rat thymocytes. J Exp Med 165:1-13 (1987). Read more (PubMed: 3098892) »

See all 2 Publications for this product

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