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Tissue/ cell preparation (Rat).Rat T cell blasts
The antibody recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labelled dendritic cells, granulocytes and microglial cells in the brain.
Our Abpromise guarantee covers the use of ab22383 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/100 - 1/200.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.
ab22383 requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
|IP||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"