FITC荧光Anti-CD53抗体[MEM-53] (ab68565)
Key features and details
- FITC Mouse monoclonal [MEM-53] to CD53
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
概述
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产品名称
FITC荧光Anti-CD53抗体[MEM-53]
参阅全部 CD53 一抗 -
描述
FITC荧光小鼠单克隆抗体[MEM-53] to CD53 -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
ab68565 reacts with CD53 exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells. This antibody reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F). -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD53. Leukocytes of patient suffering from LGL-type leukemia
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阳性对照
- Human blood cells.
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常规说明
The antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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纯度
Size exclusion -
克隆
单克隆 -
克隆编号
MEM-53 -
同种型
IgG1 -
研究领域
相关产品
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68565于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
Use neat
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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说明 |
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Flow Cyt
Use 10µl for 106 cells. Use neat
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
靶标
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功能
May be involved in growth regulation in hematopoietic cells. -
组织特异性
B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 963 Human
- Omim: 151525 Human
- SwissProt: P19397 Human
- Unigene: 443057 Human
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别名
- AI323659 antibody
- Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
- CD 53 antibody
see all
图片
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Overlay histogram showing peripheral blood lymphocytes stained with ab68565 (red line). The cells incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab68565, 1 µg/1x106 cells) for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 FITC (2 µg/1x106 cells ) for 30 min at 22°C. Acquisition of >5,000 events was performed.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab68565 被引用在 2 文献中.
- Rasmussen AM et al. Cross-linking of CD53 promotes activation of resting human B lymphocytes. J Immunol 153:4997-5007 (1994). PubMed: 7963560
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia Biol (Praha) 35:289-97 (1989). PubMed: 2534480