概述

  • 产品名称Anti-CD53抗体[MEM-53]
    参阅全部 CD53 一抗
  • 描述
    小鼠单克隆抗体[MEM-53] to CD53
  • 特异性Human CD53 antigen. This antibody also recognizes the deglycosylated antigen (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IP, WB, ICC/IF, Functional Studiesmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation (Human). (Leucocytes from a patient suffering from a LGL-type leukaemia).

  • 常规说明


    The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by protein A-affinity chromatography.
  • Primary antibody说明The antibody reacts with CD53 antigen, a glycoprotein of 32-40 kDa belonging to the tetraspans family (TM4SF). CD53 antigen is exclusivelly expressed on leucocytes (not present on platelets, red blood cells and non-hematopoietic cells). CD53 is important for signal transduction. CD53 cross-linking promotes activation of human B cells and rat macrophages.
  • 克隆单克隆
  • 克隆编号MEM-53
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use a concentration of 4 µg/ml.
IHC-Fr Use at an assay dependent concentration. Can be used to discriminate lymphomas from other tumors.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 17438075
Functional Studies Use at an assay dependent concentration. Induces activation of monocytes and B lymphocytes.

靶标

  • 功能May be involved in growth regulation in hematopoietic cells.
  • 组织特异性B-cells, monocytes, macrophages, neutrophils, single (CD4 or CD8) positive thymocytes and peripheral T-cells.
  • 序列相似性Belongs to the tetraspanin (TM4SF) family.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI323659 antibody
    • Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody
    • CD 53 antibody
    • CD53 antibody
    • CD53 antigen antibody
    • CD53 antigen, isoform CRA_b antibody
    • CD53 glycoprotein antibody
    • Cd53 molecule antibody
    • CD53 tetraspan antigen antibody
    • CD53_HUMAN antibody
    • Cell surface glycoprotein CD53 antibody
    • Leukocyte antigen MRC OX-44 antibody
    • Leukocyte surface antigen CD53 antibody
    • MOX 44 antibody
    • MOX44 antibody
    • OTTHUMP00000059505 antibody
    • Ox-44 antibody
    • OX44 antibody
    • RATOX44 antibody
    • Tetraspanin 25 antibody
    • Tetraspanin-25 antibody
    • Tetraspanin25 antibody
    • Transmembrane glycoprotein antibody
    • TSPAN 25 antibody
    • Tspan-25 antibody
    • TSPAN25 antibody
    see all

Anti-CD53 antibody [MEM-53] 图像

  • ab667 staining CD53 in human monocyte–derived macrophages by Immunocytochemistry/ Immunofluorescence. Bar 10 µm.

Anti-CD53 antibody [MEM-53] (ab667)参考文献

This product has been referenced in:
  • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . Read more (PubMed: 22634564) »
  • Ruiz-Mateos E  et al. CD63 is not required for production of infectious human immunodeficiency virus type 1 in human macrophages. J Virol 82:4751-61 (2008). ICC/IF . Read more (PubMed: 18321974) »

See all 8 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab667.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"