Anti-CD51 + CD61抗体[BV3] (ab7166)

概述

  • 产品名称Anti-CD51 + CD61抗体[BV3]
    参阅全部 CD51 + CD61 一抗
  • 描述
    小鼠单克隆抗体[BV3] to CD51 + CD61
  • 特异性This monoclonal antibody binds to human CD51 and Cd61 integrins present on human endothelium cells.
  • 经测试应用适用于: ICC/IF, IHC-P, IHC-Fr, IP, Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Rat, Chicken, Human

性能

应用

Our Abpromise guarantee covers the use of ab7166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/250.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. They are known to participate in cell adhesion as well as cell-surface mediated signalling. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD51 encodes integrin alpha chain V. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. The CD61 protein product is the integrin beta chain beta 3. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrin alpha V/beta 3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha V/beta 3 recognizes the sequence R-G-D in a wide array of ligands. The alpha V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase 2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands.
    • 细胞定位Cell Membrane; single-pass type I membrane protein.
    • 数据库链接
    • 别名
      • Antigen identified by monoclonal antibody L230 antibody
      • CD51 antibody
      • CD51 antigen antibody
      • CD61 antibody
      • CD61 antigen antibody
      • DKFZp686A08142 antibody
      • GP3A antibody
      • GPIIIa antibody
      • Integrin alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • Integrin alpha v antibody
      • Integrin beta 3 (platelet glycoprotein IIIa, antigen CD61) antibody
      • Integrin beta 3 antibody
      • Integrin beta chain beta 3 antibody
      • ITGAV antibody
      • ITGB3 antibody
      • MSK8 antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet membrane glycoprotein IIIa antibody
      • Vitronectin receptor subunit alpha antibody
      • VNRA antibody
      see all

    Anti-CD51 + CD61 antibody [BV3] 图像

    • ab7166 staining human breast cancer cells by ICC/IF. Cells were paraformaldehyde fixed for 10 minutes and blocked in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours. An Alexa Fluor® 488 conjugated goat polyclonal to mouse antibody, diluted 1/200 was used as the secondary.

      See Abreview

    • ab7166 staining CD51 and CD61 in human MeWo melanoma cells by Flow Cytometery. Cells were cultured in 24 well plates and harvested with 100 µL trypsin/ EDTA. Cells were fixed in 1:1 mixture of FACS/Flow and 4% paraformaldehyde in PBS. The primary antibody was diluted to 1 µg/ml and incubated with sample in 3% bovine serum albumin in phosphate buffer for 30 minutes at 37°C. An ab6785, FITC-conjugated goat polyclonal to mouse IgG (1/100) was used as secondary. A459 cells were used as negative control and showed no significant binding of the antibody. Different concentrations were evaluated and 1 µg/ml yield gave the best results. Blank cells were not treated with any antibodies at all, sec only cells were treated with secondary but not primary antibody.

      See Abreview

    Anti-CD51 + CD61 antibody [BV3] (ab7166)参考文献

    This product has been referenced in:
    • Koutsioumpa M  et al. Interplay between avß3 integrin and nucleolin regulates human endothelial and glioma cell migration. J Biol Chem 288:343-54 (2013). IHC-P ; Human . Read more (PubMed: 23161541) »
    • Dubiel EA  et al. In vitro morphogenesis of PANC-1 cells into islet-like aggregates using RGD-covered dextran derivative surfaces. Colloids Surf B Biointerfaces 89:117-25 (2012). IHC-P ; Human . Read more (PubMed: 21962947) »

    See all 8 Publications for this product

    Product Wall

    Thank you for your enquiry.

    I can confirm that we recommend not to dilute antibodies before storage unless is states to do so on the individual datasheet. For all antibodies, we suggest to always follow instructions provided on the individua...

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    Thank you for contacting us.

    Unfortunately, we have not mapped the epitope of ab7166 so we haveno informationif ab7166 is against the entire complex or not.


    I hope this information is helpful to you. Please do not hesitate to ...

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    Thank you for your email.

    The sensitivity of staining could be increased by;

    - using enhancers; we have DAB enhancer in catalogue, please check the datasheet http://www.abcam.com/DAB-Enhancer-ab675.html
    - Using lower dilution ...

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    Thank you for your email.

    The CD51 and CD61 target is expressed by endothelial cells, megakaryocytes, monocytes, macrophages and Neutophils which is why the staining is more in the blood vessels.

    It seems the antibody is binding to ...

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    As discussed on phone please attach images and send it to us.

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    Yes, I can confirm that this Abreview has been published. Please thank the customer for their valuable feedback.

    You can now use the discount code when the customer places an order for their next primar...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (breast cancer cell line stably expressing Integrin)
    Loading amount 60 µg
    Specification breast cancer cell line stably expressing Integrin
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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    Verified customer

    提交于 Jan 30 2012

    Thank you for contacting Abcam. I have checked our notes and it appears that ab7166 is unsuitable for western blot, while ab24694 will most probably work for western blotting, its just that we have not tested it in that application. Please let me know ...

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    Thank you for your enquiry. I would advise you to follow the storage instructions provided on the datahseet. Each product has specific instruction for shipping and storage. As our Abpromise indicates, in the event that a product is not functioning ...

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    Thank you for contacting us. I have looked at a number of candidates for cell surface markers for HeLa cells, but I am not aware of any that are unique to HeLa cells, if you are trying to separate them from a mixed population. The following pap...

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