The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab126026 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Human CD49b is an integrin alpha 2 subunit that forms a heterodimer with beta 1 integrin and functions as an adhesion molecule. CD49b is a 160 kDa glycoprotein that noncovalently associates with the 130 kDa integrin beta 1 subunit (CD29) to form glycoprotein Ia/IIa (when expressed on platelets), and very late (activation) antigen 2 ('VLA2') when found on T cells. CD49b is expressed by platelets, approximately 50% of monocytes, long term cultivated T cells and most adherent cell lines.
Integrin alpha 2/beta 1 is a receptor for laminin, collagen, collagen C propeptides, fibronectin and E cadherin. It recognizes the proline hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.
Cell Membrane; Single pass type I membrane protein.