Anti-CD47抗体[MEM-122] (ab9089)

概述

  • 产品名称Anti-CD47抗体[MEM-122]
    参阅全部 CD47 一抗
  • 描述
    小鼠单克隆抗体[MEM-122] to CD47
  • 特异性Integrin Associated Protein
  • 经测试应用适用于: Flow Cyt, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human, Pig, African Green Monkey
    预测可用于: Non Human Primates
  • 免疫原

    COS-7 (African green monkey) cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明Purified from ascites by precipitation methods and size-exclusion chromatography.
  • 克隆单克隆
  • 克隆编号MEM-122
  • 同种型IgM
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.1-1µg for 106 cells. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Use under non reducing condition.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
  • 组织特异性Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen identified by monoclonal antibody 1D8 antibody
    • Antigenic surface determinant protein OA3 antibody
    • CD 47 antibody
    • CD47 antibody
    • CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
    • CD47 antigen antibody
    • CD47 glycoprotein antibody
    • CD47 molecule antibody
    • CD47_HUMAN antibody
    • IAP antibody
    • Integrin Associated Protein antibody
    • Integrin associated signal transducer antibody
    • Integrin-associated protein antibody
    • Leukocyte surface antigen CD47 antibody
    • MER 6 antibody
    • MER6 antibody
    • OA 3 antibody
    • OA3 antibody
    • OTTHUMP00000041152 antibody
    • OTTHUMP00000041153 antibody
    • Protein MER6 antibody
    • Rh related antigen antibody
    see all

Anti-CD47 antibody [MEM-122] 图像

  • Human peripheral blood lymphocytes stained with ab9089 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab9089, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

Anti-CD47 antibody [MEM-122] (ab9089)参考文献

ab9089 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

As we discussed on the phone, the immunogen sequences for ab53444, ab9089, and ab3283 are unknown due to all 3 immunogens arising from tissue and cell preparations.

For CD47 (ab9089 and ab3283) the huma...

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Thanks for your email. Ab9089 does cross-react with human and has been tested for application in IHC-frozen sections. If you have any more questions, please contact us again.

Thank you for your enquiry. For ab3283, the information that we regarding the epitope is that it is the Ig domain. For ab9089, the epitope has not yet been mapped. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"