Anti-CD47抗体[B6H12.2] (ab3283)


  • 产品名称Anti-CD47抗体[B6H12.2]
    参阅全部 CD47 一抗
  • 描述
    小鼠单克隆抗体[B6H12.2] to CD47
  • 经测试应用适用于: Flow Cyt, Functional Studies, ICC, IP, ICC/IF, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Intact CD47 purified from placenta.

  • 表位Ig domain.
  • 阳性对照
    • Tonsil.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.40
    Constituents: 0.0268% PBS, 0.2% BSA
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号B6H12.2
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab3283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Functional Studies Use at an assay dependent concentration.

This antibody blocks the binding of SIRP alpha (Yoshida, et al.; Latour et al.) and inhibits in assays where CD47-integrin association is required.

ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

(native, with protein G)

ICC/IF Use at an assay dependent concentration. PubMed: 20923773
IHC-Fr Use at an assay dependent concentration. PubMed: 22451919
WB Use at an assay dependent concentration. Detects a band of approximately 49 kDa (predicted molecular weight: 35 kDa).


  • 功能Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.
  • 组织特异性Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.
  • 序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Antigen identified by monoclonal antibody 1D8 antibody
    • Antigenic surface determinant protein OA3 antibody
    • CD 47 antibody
    • CD47 antibody
    • CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
    • CD47 antigen antibody
    • CD47 glycoprotein antibody
    • CD47 molecule antibody
    • CD47_HUMAN antibody
    • IAP antibody
    • Integrin Associated Protein antibody
    • Integrin associated signal transducer antibody
    • Integrin-associated protein antibody
    • Leukocyte surface antigen CD47 antibody
    • MER 6 antibody
    • MER6 antibody
    • OA 3 antibody
    • OA3 antibody
    • OTTHUMP00000041152 antibody
    • OTTHUMP00000041153 antibody
    • Protein MER6 antibody
    • Rh related antigen antibody
    • Surface antigen identified by monoclonal antibody 1D8 antibody
    see all

Anti-CD47 antibody [B6H12.2] 图像

  • ab3283 at 1µg/ml staining CD47 from human OVCA3 cells by ICC. The cells were methanol fixed, blocked with 3% serum and incubated with the primary antibody for 30 minutes. A biotinylated horse anti-mouse IgG was used as the secondary antibody.

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  • ab3283 staining CD47 in HUVEC cells by Immunocytochemistry/ Immunofluorescence.
    The cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.25% Triton X-100 for 5 minutes and blocked with 1% BSA in 0.1% Tween 20 and PBS for 1 hour at room temperature. The cells were incubated in a mixture of two primary antibodies ab3283 and a rabbit anti-human VEGFR2 overnight at 4 °C. The cells were then incubated with a mixture of two secondary antibodies (Alexa Fluor 488 anti-rabbit and Alexa Fluor 594 anti-mouse) in 1% BSA for 1 hour at room temperature in the dark. Counterstained with DAPI.
  • Overlay histogram showing peripheral blood lymphocytes stained with ab3283 (red line). The cells were incubated with the antibody (ab3283, 1µg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/200 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

  • Anti-CD47 antibody [B6H12.2] (ab3283) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    Exposure time : 4 minutes
    CD47 contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

Anti-CD47 antibody [B6H12.2] (ab3283)参考文献

This product has been referenced in:
  • Kaur S  et al. CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells. Matrix Biol 37:49-59 (2014). WB ; Cow . Read more (PubMed: 24887393) »
  • Edris B  et al. Antibody therapy targeting the CD47 protein is effective in a model of aggressive metastatic leiomyosarcoma. Proc Natl Acad Sci U S A 109:6656-61 (2012). IHC-Fr . Read more (PubMed: 22451919) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (multiple myeloma cell line xenograft)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization No
Specification multiple myeloma cell line xenograft
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: 23°C
Fixative 10% NBF

Abcam user community

Verified customer

提交于 Oct 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human)
Gel Running Conditions Reduced Denaturing
Loading amount 30 µg
Treatment alpha synuclein
Specification human
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Ms. Sehee Oh

Verified customer

提交于 Jul 13 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Sample Cow Serum (FBS)
Specification FBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Jun 11 2014

These products are all mouse monoclonal against CD-47. It appears that ab3283 and ab140325 are the same clone. Clone B6H12.2 is first described in: The Role of CD47 in Neutrophil Transmigration increased rate of migration correlates with increased ...

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Thank you for contacting Abcam.

As we discussed on the phone, the immunogen sequences for ab53444, ab9089, and ab3283 are unknown due to all 3 immunogens arising from tissue and cell preparations.

For CD47 (ab9089 and ab3283) the huma...

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The protocol for incubating the antibody with the protein is the same as incubating the antibody with peptide. Ideally, you want to inucbate the antibody with a molar excess of 50-fold more protein per antibody binding site to increase the probability ...

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Thank you for contacting us.

The antibody ab3283 is raised against full-length CD47 and the epitope the antibody recognizes has not been mapped, so there is no blocking peptide available. That is also the case for several other CD47 antibodi...

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Expiration date: November 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab3283 in IHC-P. This code will give you: 1 freeprimary antibody before the expiration date. To redeem this off...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

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