使用敲除细胞株进行验证RabMAb

Anti-CD46抗体[EPR4014] (ab108307)

概述

  • 产品名称Anti-CD46抗体[EPR4014]
    参阅全部 CD46 一抗
  • 描述
    兔单克隆抗体[EPR4014] to CD46
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Rabbit, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human CD46.

  • 阳性对照
    • Molt-4, Jurkat, HeLa, and K562 cell lysates; Human kidney and Tonsil tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-CD46 antibody (HRP) [EPR4014] (ab196863)

    Anti-CD46 antibody (Alexa Fluor® 488) [EPR4014] (ab197489)

    Anti-CD46 antibody (Alexa Fluor® 647) [EPR4014] (ab196862)

性能

应用

Our Abpromise guarantee covers the use of ab108307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.
    • 组织特异性Expressed by all cells except erythrocytes.
    • 疾病相关Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations.
    • 序列相似性Contains 4 Sushi (CCP/SCR) domains.
    • 结构域Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b.
    • 翻译后修饰N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
      Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
      In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck.
    • 细胞定位Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AHUS2 antibody
      • Antigen defined by monoclonal antibody TRA 2 10 antibody
      • Antigen identified by monoclonal antibody TRA 2 10 antibody
      • CD46 antibody
      • CD46 antigen antibody
      • CD46 antigen complement regulatory protein antibody
      • CD46 molecule antibody
      • CD46 molecule complement regulatory protein antibody
      • Complement membrane cofactor protein antibody
      • MCP antibody
      • MCP_HUMAN antibody
      • Measles virus receptor antibody
      • membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen) antibody
      • Membrane cofactor protein antibody
      • MGC26544 antibody
      • MIC10 antibody
      • TLX antibody
      • TRA2.10 antibody
      • Trophoblast leucocyte common antigen antibody
      • Trophoblast leukocyte common antigen antibody
      • Trophoblast lymphocyte cross reactive antigen antibody
      see all

    Anti-CD46 antibody [EPR4014] 图像



    • Predicted band size : 44 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: CD46 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: K562 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108307 observed at 50-70 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab108307 was shown to specifically react with  when CD46 knockout samples were used. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. ab108307 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded Human tonsil tissue
    • ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded Human kidney tissue
    • All lanes : Anti-CD46 antibody [EPR4014] (ab108307) at 1/1000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 44 kDa
    • ab108307 showing positive staining in Normal placenta tissue.

    • ab108307 showing positive staining in Normal uterus tissue.

    • ab108307 showing positive staining in Thyroid gland carcinoma tissue.

    • ab108307 showing positive staining in Colonic adenocarcinoma tissue.

    • ab108307 showing positive staining in Breast carcinoma tissue.

    • ab108307 showing positive staining in Normal breast tissue.

    Anti-CD46 antibody [EPR4014] (ab108307)参考文献

    This product has been referenced in:
    • Galanis E  et al. Oncolytic measles virus expressing the sodium iodide symporter to treat drug-resistant ovarian cancer. Cancer Res 75:22-30 (2015). IHC, IF ; Human . Read more (PubMed: 25398436) »
    • Scambi C  et al. The local complement activation on vascular bed of patients with systemic sclerosis: a hypothesis-generating study. PLoS One 10:e0114856 (2015). IHC-P ; Human . Read more (PubMed: 25658605) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your inquiry.

    As far as we are aware, the epitope has not been mapped yet.

    Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

    We ...

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    Thank you for contacting us.

    I do not see an antibody in our catalogue that I am confident will react with mouse CD46. The antibody ab72836 appears to detect in a western blot of rat samples a protein a protein that is the expected molecular...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rabbit Tissue sections (tonsil, intestine)
    Specification tonsil, intestine
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris buffer (Ventana)
    Permeabilization No
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    提交于 Mar 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"