PE Anti-CD45RO抗体[UCHL1] (ab77217)
Key features and details
- PE Mouse monoclonal [UCHL1] to CD45RO
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
概述
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产品名称
PE Anti-CD45RO抗体[UCHL1]
参阅全部 CD45RO 一抗 -
描述
PE小鼠单克隆抗体[UCHL1] to CD45RO -
宿主
Mouse -
偶联物
PE. Ex: 488nm, Em: 575nm -
特异性
Recognizes CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes. -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD45RO. Human IL-2 dependent T cells
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阳性对照
- Flow Cyt: Human peripheral blood lymphocytes.
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常规说明
The conjugate is purified by size exclusion chromatography and adjusted for direct use in flow cytometry.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.88% Sodium chloride, 0.12% Monobasic dihydrogen sodium phosphate -
Concentration information loading...
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纯度
Affinity purified -
克隆
单克隆 -
克隆编号
UCHL1 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 5µl for 105-8 cells.
Use 5µl in a final volume of 100µl. ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 5µl for 105-8 cells. Use 5µl in a final volume of 100µl. ab91363 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. -
疾病相关
Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Contains 2 fibronectin type-III domains.
Contains 2 tyrosine-protein phosphatase domains. -
结构域
The first PTPase domain interacts with SKAP1. -
翻译后修饰
Heavily N- and O-glycosylated. -
细胞定位
Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 5788 Human
- Omim: 151460 Human
- SwissProt: P08575 Human
- Unigene: 654514 Human
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别名
- B220 antibody
- CD45 antibody
- cd45 antigen antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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