Anti-CD45RO抗体[UCH-L1] (ab23)

概述

  • 产品名称Anti-CD45RO抗体[UCH-L1]
    参阅全部 CD45RO 一抗
  • 描述
    小鼠单克隆抗体[UCH-L1] to CD45RO
  • 特异性UCH-L1 may identify abnormalities of functional T-cell subsets in immune disorders and can be used in tumour diagnosis by FACS or immunohistology. Very useful in studies of human T cell function and ontogeny.
  • 经测试应用适用于: Flow Cyt, IP, WB, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Cultured T cells from an IL-2-dependent T-cell line (CA1) prepared from human peripheral blood activated with influenza virus.

  • 阳性对照
    • This antibody gave a positive signal in human tonsil and thymus tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab23 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 疾病相关Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
    Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域The first PTPase domain interacts with SKAP1.
  • 翻译后修饰Heavily N- and O-glycosylated.
  • 细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B220 antibody
    • CD45 antibody
    • cd45 antigen antibody
    • CD45R antibody
    • GP180 antibody
    • L-CA antibody
    • lca antibody
    • Leukocyte common antigen antibody
    • LY 5 antibody
    • LY5 antibody
    • Protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45RO antibody [UCH-L1] 图像

  • ab23 (1µg/ml) staining CD45RO in human tonsil.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • All lanes : Anti-CD45RO antibody [UCH-L1] (ab23) at 5 µg/ml

    Lane 1 : Human tonsil normal tissue lysate - total protein (ab29615)
    Lane 2 : Human thymus tissue lysate - total protein (ab30146)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 131 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Overlay histogram showing HeLa cells stained with ab23 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-CD45RO antibody [UCH-L1] (ab23)参考文献

This product has been referenced in:
  • Tarhini AA  et al. Immune monitoring of the circulation and the tumor microenvironment in patients with regionally advanced melanoma receiving neoadjuvant ipilimumab. PLoS One 9:e87705 (2014). IHC-P ; Human . Read more (PubMed: 24498358) »
  • Zlobec I  et al. Next-generation tissue microarray (ngTMA) increases the quality of biomarker studies: an example using CD3, CD8, and CD45RO in the tumor microenvironment of six different solid tumor types. J Transl Med 11:104 (2013). IHC ; Human . Read more (PubMed: 23627766) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization No
Specification Lymph node
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative Paraformaldehyde
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Verified customer

提交于 Sep 30 2015


The concentration for this lot is 1 mg/ml. We haven't specified a dilution range for this product in IHC-P however on the datasheet, IHC-P was successfully performed using 1 ug/ml therefore a 1/1000 dilution. I would suggest starting with this dil...

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Thank you for contacting us. No, ab23 does not contain any BSA, the antibody is in PBS only.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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We have not had any reports of this antibody being used with cynamologous and the nature of the epitope is unknown. A reference to the immunodetection of CD45RO in cynaomolgous as shown below: Li SL et al, Res Virol 1991 Mar-Jun;142(2-3):173-82

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"