Anti-CD45RA抗体[MRC OX-33] (ab33933)

概述

  • 产品名称Anti-CD45RA抗体[MRC OX-33]
    参阅全部 CD45RA 一抗
  • 描述
    小鼠单克隆抗体[MRC OX-33] to CD45RA
  • 特异性This antibody is directed against a high molecular weight band of the leucocyte common antigen (LCA).
  • 经测试应用适用于: Flow Cyt, IHC-Fr, IHC-Pmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Purified spleen leucocyte common antigen.

  • 常规说明


    Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line. This clone has been described reacting with paraffin embedded material following PLP fixation (see Whiteland et al., 1995). It only labels B cells among thoracic duct lymphocytes, with little labelling in bone marrow and none on thymocytes (Barclay et al., 1987).

性能

应用

Our Abpromise guarantee covers the use of ab33933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.





ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.
  • 疾病相关Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis
  • 序列相似性Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.
  • 结构域The first PTPase domain interacts with SKAP1.
  • 翻译后修饰Heavily N- and O-glycosylated.
  • 细胞定位Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B220 antibody
    • CD45 antibody
    • CD45 antigen antibody
    • CD45R antibody
    • EC 3.1.3.48 antibody
    • GP180 antibody
    • L-CA antibody
    • LCA antibody
    • Leukocyte common antigen antibody
    • Loc antibody
    • Ly-5 antibody
    • LY5 antibody
    • Ly5, homolog of antibody
    • Lymphocyte antigen 5 antibody
    • Lyt-4 antibody
    • Protein tyrosine phosphatase, receptor type, C antibody
    • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
    • Ptprc antibody
    • PTPRC_HUMAN antibody
    • Receptor-type tyrosine-protein phosphatase C antibody
    • RT7 antibody
    • T200 antibody
    • T200 glycoprotein antibody
    • T200 leukocyte common antigen antibody
    see all

Anti-CD45RA antibody [MRC OX-33] (ab33933)参考文献

This product has been referenced in:
  • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »
  • Whiteland JL  et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Read more (PubMed: 7868861) »

See all 2 Publications for this product

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Thank you for contacting us.

I have emailed the lab to see if they have an IHC-P image they can share, and will let you know when I hear back from them.

The reference I had mentioned is as follows:

Whiteland JL et al. I...

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Thank you for your recent telephone enquiry. I have obtained the following general testing method from the originator of ab33933 and ab33934. Please note that this protocol is a guideline only and may need to be optimized in your laboraotry. I h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"